Cultured BMMs were detached as described by Han et al. [14 (link)]. The BMMs were resuspended at 1.0 × 106 cells/1 ml in PBS and then incubated with FITC-anti-mouse CD86 (clone GL1, TONBO Biosciences, San Diego, CA), PE-anti-mouse CD80 (clone 16-10A1, TONBO), Alexa Fluor® 647-anti-mouse CD206 (clone MR5D3, BD Pharmingen). For immunoprofiling of tumor-bearing mice, single cell suspensions of Hepa1-6 tumors or the draining lymph nodes (dLN) at 1 × 106 cells/sample were immunostained with a combination of some of the following antibodies, such as FITC-anti-mouse CD4 (clone GK1.5, Tonbo), PE-anti-mouse CD11b (clone M1/70, BD), PerCP-Cy5-anti-mouse-B220 (clone RA3-6B2, Tonbo), APC-anti-mouse-CD11c (clone HL3, BD), eFluor450-anti-mouse CD45 (clone 30-F11, eBioscience), APC-Cy7-anti-mouse-CD8 (clone 53-6.7, Tonbo), eFluor450-anti-mouse CD44 (clone IM7, eBioscience), APC-anti-mouse CD62L (clone MEL-14, eBioscience), eFluor450-anti-mouse CD8 (clone 53-6.7, eBioscience), and eFluor660-anti-mouse CD107a (clone 1D4B, eBioscience). Data of the stained samples were acquired using an LSR II flow cytometer (BD) and analyzed using the software FlowJo (Tree Star Inc., Ashland, OR).
Percp cy5 anti mouse b220 clone ra3 6b2
Manufactured by Cytek Biosciences
PerCP-Cy5-anti-mouse-B220 (clone RA3-6B2) is a fluorochrome-conjugated antibody that binds to the B220 antigen expressed on mouse B cells. It can be used in flow cytometry applications for the identification and analysis of B cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Apc anti mouse cd11c clone hl3, by BD (2 mentions)
Lsrii flow cytometer, by BD (2 mentions)
Efluor450 anti mouse cd45 clone 30 f11, by Thermo Fisher Scientific (2 mentions)
Fitc anti mouse cd86 clone gl1, by Cytek Biosciences (2 mentions)
Pe anti mouse cd80 clone 16 10a1, by Cytek Biosciences (2 mentions)
Fitc anti mouse cd4 clone gk1, by Cytek Biosciences (2 mentions)
Apc cy7 anti mouse cd8 clone 53 6, by Cytek Biosciences (2 mentions)
2 protocols using percp cy5 anti mouse b220 clone ra3 6b2
1
Multicolor Flow Cytometry of BMMs and Tumor Samples
2
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DCs suspended in FACS buffer (PBS containing 0.5% BSA and 0.05% NaN3) were blocked with 2% mouse serum on ice for 20 min and stained with various combinations of fluorophore-conjugated antibodies against human or mouse DC surface markers on ice for 30 min in the dark. Mouse DCs were stained with FITC-anti-mouse CD86 (clone GL1, TONBO Biosciences, San Diego, CA), PE-anti-mouse CD80 (clone 16-10A1, TONBO), Pacific Blue-anti-mouse CD83 (clone Michel-19, BD), and APC-anti-mouse I-A/E (clone M5/114.15.2, eBioscience). Single LLC tumor cell suspensions were stained with FITC-anti-mouse CD4 (clone GK1.5, Tonbo), PerCP-Cy5-anti-mouse-B220 (clone RA3-6B2, Tonbo), APC-anti-mouse-CD11c (clone HL3, BD), eFluor450-anti-mouse CD45 (clone 30-F11, eBioscience), and APC-Cy7-anti-mouse-CD8 (clone 53 − 6.7, Tonbo). Data of the stained samples were acquired using an LSR II flow cytometer (BD) and analyzed using the software FlowJo.
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