Glycocholic acid

Manufactured by Steraloids

Sourced in United States

Glycocholic acid is a bile acid derivative that is commonly used as a biochemical reagent in laboratory settings. It serves as a critical component in various analytical and research applications.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using glycocholic acid

Baicalin and Bile Acid Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Baicalin (>98%) was purchased from Dalian Meilun Biotechnology Co., Ltd (Meilunbio, Dalian, China). EE was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Standards for BAs for cholic acid (CA), deoxycholic acid (DCA), chenodeoxycholic acid (CDCA), β-muricholic acid (β-MCA), taurocholic acid (TCA), taurodeoxycholic acid (TDCA), tauromuricholic acid (T-MCA), taurochenodeoxycholic acid (TCDCA), glycocholic acid (GCA), glycoursodesoxycholic acid (GUDCA), glycohyocholic acid (GHDCA), glycochenodeoxycholic acid (GCDCA), taurohyocholic acid (THDCA), glycodeoxycholic acid (GDCA), and interior label d4-glycochenodeoxycholic acid (d4-GCDCA) were obtained from Steraloids (Newport, RI, USA).
Antibodies directed against FXR, CAR, PXR, VDR, cholesterol 7a-hydroxylase (CYP7A1), and sterol 27-hydroxylase (CYP27A1) were purchased from Absin Biochemical Company (Shanghai, China). Antibodies directed against Sirt1, HNF-1α, tumor necrosis factor α (TNF-α), interleukin 1 beta (IL-1β), IL-6, and NF-κB were purchased from Cell Signaling Technology (Beverly, MA, USA). An antibody against BSEP was obtained from Santa Cruz Biotechnology (CA, USA). And an antibody against MRP2 was obtained from Abcam (Cambridge, UK).

Yang J., Xiang D., Xiang D., He W., Liu Y., Lan L., Li G., Jiang C., Ren X., Liu D, & Zhang C. (2020). Baicalin Protects Against 17α-Ethinylestradiol-Induced Cholestasis via the Sirtuin 1/Hepatic Nuclear Receptor-1α/Farnesoid X Receptor Pathway. Frontiers in Pharmacology, 10, 1685.

+ Open protocol

+ Expand

Synthesis and Characterization of Bile Acid Derivatives

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Deoxy cholic acid (DCA), chenoDeoxy cholic acid (CDCA), cholic acid (CA), α-muricholic acid, β-muricholic acid, glycoDeoxy cholic acid (GDCA), glycochenoDeoxy cholic acid (GCDCA), and glycocholic acid (GCA) were obtained from Steraloids, Inc. Bile acid structures are shown in fig. S1. Bile acid A (37 ), 5-cholanic acid-3α,4β,7α-triol (7)(38 (link)), and AMPP (39 (link)) were synthesized according to published procedures. N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), 4-(dimethylamino)pyridine (DMAP), diethylamine, N,N-dimethylformamide, trisodium citrate, sodium hydroxide (NaOH), chloroform, Dulbecco’s modified Eagle’s medium, fetal calf serum, and penicillin G and streptomycin sulfate were obtained from Sigma–Aldrich. HPLC solvents (methanol and acetonitrile) were HPLC grade and were purchased from EMD Chemicals. Milli-Q ultrapure water was prepared in-house with a Milli-Q Integral Water Purification System.

Jiang X., Sidhu R., Mydock L., Hsu F.F., Covey D.F., Scherrer D.E., Earley B., Gale S.E., Farhat N.Y., Porter F.D., Dietzen D.J., Orsini J.J., Berry-Kravis E., Zhang X., Reunert J., Marquardt T., Runz H., Giugliani R., Schaffer J.E, & Ory D.S. (2016). Development of a Bile Acid-Based Newborn Screen for Niemann-Pick C Disease. Science translational medicine, 8(337), 337ra63.

+ Open protocol

+ Expand

Quantifying Bile Acid Profiles

Check if the same lab product or an alternative is used in the 5 most similar protocols

BA standards including cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA) and hyodeoxycholic acid (HDCA) were purchased from Sigma-Aldrich Chemicals Co (Steinheim, Germany). α-muricholic acid (α-MCA), β-muricholic acid (β-MCA), ω-muricholic acid (ω-MCA), tauro-cholic acid (T-CA), tauro-chenodeoxycholic acid (T-CDCA), tauro-deoxycholic acid (T-DCA), tauro-lithocholic acid (T-LCA), tauro-ursodeoxycholic acid (T-UDCA), tauro-α-muricholic acid (T-α-MCA), tauro- β-muricholic acid (T-β-MCA), glyco-cholic acid (G-CA), glyco-chenodeoxycholic acid (G-CDCA), glyco-deoxycholic acid (G-DCA), glyco-lithocholic acid (G-LCA), glyco-ursodeoxycholic acid (G-UDCA) and deuterated internal standards (IS) lithocholic acid-2,2,4,4-D4 (LCA-D4), deoxycholic acid-2,2,4,4-D4 (DCA-D4) and glycochenodeoxycholic acid-2,2,4,4-D4 (GCDCA-D4) were obtained from Steraloids, Inc. (Newport, RI, USA). Methanol HPLC grade was from Merck (Darmstadt, Germany). Ultra-pure reagent water was purified by a Milli-Q gradient system (Millipore, Bedford, MA, USA). Acetonitrile LC-MS grade was from VWR and formic acid LC-MS grade was purchased from Sigma Aldrich Chemicals Co.

Ghaffarzadegan T., Essén S., Verbrugghe P., Marungruang N., Hållenius F.F., Nyman M, & Sandahl M. (2019). Determination of free and conjugated bile acids in serum of Apoe(−/−) mice fed different lingonberry fractions by UHPLC-MS. Scientific Reports, 9, 3800.

+ Open protocol

+ Expand

Analytical Standards Procurement and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ephedrine hydrochloride was purchased by National Institute for the Control of Pharmaceutical and Biological products of China (Beijing, China). Sinapine thiocyanate, tetrahydropalmatine, baicalin and asarinin were purchased from Chengdu Chroma-Biotech Co. Ltd. (Chengdu, China). All compounds except Ephedrine hydrochloride had a purity of 98%; the purity of Ephedrine hydrochloride was 99%.
Glycocholic acid was purchased from Steraloids (Newport, RI, USA) while nor epinephrine, epinephrine, alanine serine and valine were obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC-graded acetonitrile and methanol were obtained from Fisher Scientific (Hampton, NH, USA). Cholic acid D4 and formic acid were purchased from Cambridge Isotope Laboratory Inc. (Cambridge, UK) and Sigma-Aldrich (St. Louis, MO, USA), respectively. Water was purified in-house using a Milli-Q Advantage A10 water purification system (Millipore, Bedford, MA, USA).

Xiang S.J., Li M.H., Chan C.O., Shen Q., Chen S.B., An B.C., Yuen A.C., Wu W.F., Tang H.H., Cao S.W., Ruan S.F., Wang Z.X., Weng L.D., Zhu H.X., Chen H.J., Wong M.Y., Zhang Y., Mok D.K, & Liu Q. (2019). Altered metabolites in guinea pigs with allergic asthma after acupoint sticking therapy: New insights from a metabolomics approach. Phytomedicine : international journal of phytotherapy and phytopharmacology, 54.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!