Rpmi 1640

Manufactured by Atlanta Biologicals

Sourced in United States, Gabon

RPMI 1640 is a cell culture medium widely used in biomedical research. It is a balanced salt solution that provides essential nutrients and growth factors to support the growth and maintenance of a variety of cell types, including mammalian cells, primary cells, and immortalized cell lines.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (20 mentions) Hepes, by Thermo Fisher Scientific (3 mentions) Penicillin, by Thermo Fisher Scientific (3 mentions) Streptomycin, by Thermo Fisher Scientific (3 mentions) Mda mb 231, by Atlanta Biologicals (2 mentions) Phytohemagglutinin (pha), by Merck Group (2 mentions) Nec 1, by Merck Group (2 mentions) P4543, by Merck Group (2 mentions) Neurocult ns a proliferation kit, by STEMCELL (2 mentions) Mycoalert plus mycoplasma detection kit, by Lonza (2 mentions) 2 mercaptoethanol, by Merck Group (2 mentions) Universal mycoplasma detection kit, by American Type Culture Collection (2 mentions) Penicillin, by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) Anti cd28, by BioLegend (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Celltiter 96 non radioactive cell proliferation assay kit, by Promega (1 mentions) Jzl184, by Cayman Chemical (1 mentions)

Close spelling variants of this product

RPMI 1640 media RPMI-1640 medium

26 protocols using rpmi 1640

Endocannabinoid Receptor Modulation in Cell Proliferation

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The following were purchased from Cayman (Ann Arbor, MI): 2-AG; the endocannabinoid receptor inhibitors, SR141716A (Rimonabant, CB1 inverse agonist), AM251 (CB1 antagonist) and SR144528 (CB2 inverse agonist); the endocannabinoid receptor activators, CP47497 (CB1 agonist), AM1241 (CB2 agonist), Win- 55–212-2 (CB1 and CB2 agonist) and CP55940 (CB1 and CB2 agonist); the selective and nonselective inhibitors for cyclooxygenase-1 and -2 (COX-1, COX-2), SC-560 (selective for COX-1), CAY10404 (selective for COX- 2), Ibuprofen (nonselective for COX-1 and COX-2); and the selective inhibitors for hydrolases of 2-AG, JZL 184 (selective for enzyme monoacylglycerol lipase, MAGL. Anti-bromodeoxyurine (BrdU) antibody was purchased from Roche Applied Science (Indianapolis, IN). The CellTiter 96 Non-Radioactive Cell proliferation Assay Kit™ was purchased from Promega (Madison, WI). Charcoal/dextran-treated fetal bovine serum (CFBS) was purchased from Hyclone (Logan, UT). DMEM, RPMI-1640, antibiotics (penicillin and streptomycin), and fetal bovine serum (FBS) were purchased from Atlanta Biologicals (Lawrenceville, GA).

Zhang J., Medina-Cleghorn D., Bernal-Mizrachi L., Bracci P.M., Hubbard A., Conde L., Riby J., Nomura D.K, & Skibola C.F. (2016). The potential relevance of the endocannabinoid, 2-arachidonoylglycerol, in diffuse large B-cell lymphoma. Oncoscience, 3(1), 31-41.

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Transduction of HER2+ Breast Cancer Cells

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Human HER2-amplified breast cancer BT474 cells (ATCC) were cultured in RPMI 1640 supplemented with 10% FBS (Atlanta Biologicals, Flowery Branch, GA, USA). Human HER2-amplified MDA-MB-361 cells (ATCC) were cultured in DMEM/F12 supplemented with 10% FBS. BT474 and MDA-MB-361 cells were transduced with an expression cassette encoding Gaussia luciferase (Gluc) and green fluorescent protein (GFP), as previously described.⁶

Askoxylakis V., Ferraro G.B., Badeaux M., Kodack D.P., Kirst I., Shankaraiah R.C., Wong C.S., Duda D.G., Fukumura D, & Jain R.K. (2019). Dual endothelin receptor inhibition enhances T-DM1 efficacy in brain metastases from HER2-positive breast cancer. NPJ Breast Cancer, 5, 4.

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Cytogenetically Tested Cell Line Maintenance

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All cell lines were purchased from the American Type Culture Collection and were cytogenetically tested and authenticated before being frozen. Each vial of frozen cells was thawed and maintained in culture for a maximum of 8 weeks. Enough frozen vials were available for each cell line to ensure that all cell-based experiments were conducted on cells that had been tested and in culture for 8 weeks or less. The human erythroleukemia (HEL) cell line was cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals, Flowery Branch, GA) and 1% antibiotic-antimycotic (Thermo Fisher Scientific Inc., Waltham, MA). HaCaT skin keratinocytes and A431 human epidermoid carcinoma cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Inc.) supplemented with 10% FBS and 1% antibiotic-antimycotic. JB6 P+ mouse skin epidermal cells were cultured in minimum essential medium (MEM) supplemented with 5% FBS and 1% antibiotic-antimycotic.

Kim J.E., Son J.E., Jeong H., Joon Kim D., Seo S.K., Lee E., Lim T.G., Kim J.R., Chen H., Bode A.M., Lee K.W, & Dong Z. (2015). A novel cinnamon-related natural product with Pim-1 inhibitory activity inhibits leukemia and skin cancer. Cancer research, 75(13), 2716-2728.

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Lentivirus Production for LNCaP Cell Line

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The androgen-dependent human prostate carcinoma cell line LNCaP-FGC (ATCC CRL-1740) was cultured in RPMI-1640 supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA) at 37°C in 5% CO₂. The LV shuttle vector, LV-SFFVEGFP, has self-inactivating long terminal repeats (LTRs), an internal spleen focus-forming virus promoter driving EGFP expression, and R6Kγ origin of replication and a neomycin phosphotransferase gene. Vesicular stomatitis virus glycoprotein pseudotyped vector stocks were made by PEI-mediated transfection of HEK-293T cells as previously described
[64 (link)]. Functional titers were determined by transduction of HT-1080 fibrosarcoma cells. Cells were cultured for 14 days post vector exposure prior to use in experiments.

Schinke E.N., Bii V., Nalla A., Rae D.T., Tedrick L., Meadows G.G, & Trobridge G.D. (2014). A novel approach to identify driver genes involved in androgen-independent prostate cancer. Molecular Cancer, 13, 120.

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Evaluation of B. abortus Strains

Cited 2 times

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RAW264.7 cells (American Type Culture Collection, Manassas, VA) and human macrophages were used to evaluate znBAZ survival in comparison to wt B. abortus strain 2308, RB51 vaccine, and the single ΔznuA B. abortus mutant. Human macrophages were isolated as previously described [18 (link)], and approval for use of human peripheral blood was obtained from the University of Florida Institutional Review Board. Infection conditions were identical to those previously described [15 (link), 16 (link)]. After overnight culture in complete medium (CM; RPMI 1640, 10% fetal bovine serum [Atlanta Biologicals, GA], 10 mM HEPES buffer, 10 mM nonessential amino acids, 10 mM sodium pyruvate), cells were infected with a bacteria-to-macrophage ratio of 30:1 for 1 h at 37°C. Wells were washed twice with CM without antibiotics and then incubated with 50 μg/ml of gentamicin (Life Technologies) for 30 min at 37°C. After washing twice, fresh CM without antibiotics (1.0 ml/well) was added, and cells were incubated for an additional 4, 24, or 48 h.

Yang X., Clapp B., Thornburg T., Hoffman C, & Pascual D.W. (2016). Vaccination with a ΔnorD ΔznuA Brucella abortus mutant confers potent protection against virulent challenge. Vaccine, 34(44), 5290-5297.

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Culturing Breast Cell Lines for Cancer Research

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MCF10A human breast epithelial cells were maintained in Dulbecco’s modified Eagle medium (DMEM)/Ham’s F-12 (50/50) medium supplemented with epidermal growth factor 20 ng/mL, insulin

10 μ g / mL

, hydrocortisone

0.5 μ g / mL

, cholera toxin 100 ng/mL, 5% horse serum (Atlanta Biologicals), and 1% antibiotics and antimycotics (Mediatech, Manassas, Virginia). Triple-negative breast tumor cell lines (HCC1395, BT-20, MDA-MB-231, and Hs578T) were cultured in RPMI 1640 (HCC1395), EMEM (BT-20), or DMEM (MDA-MB-231, Hs578T) medium supplemented with 9% fetal bovine serum (Atlanta Biologicals) and 1% antibiotics and antimycotics. All cell lines were purchased from American Type Culture Collection (ATCC, Manassas, Virginia). Cells were maintained at 37°C in a cell culture incubator with 5%

{CO}_{2}

Palasuberniam P., Kraus D., Mansi M., Howley R., Braun A., Myers K.A, & Chen B. (2021). Small molecule kinase inhibitors enhance aminolevulinic acid-mediated protoporphyrin IX fluorescence and PDT response in triple negative breast cancer cell lines. Journal of Biomedical Optics, 26(9), 098002.

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Differentiation of THP-1 Monocytes to Macrophages

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THP-1 monocytes were obtained from ATCC and maintained in growth media (RPMI 1640 supplemented with10% FBS (Atlanta Biologicals), 50units/mL penicillin, 50µg/mL streptomycin, 10mM HEPES, pH 7.4, 2mM glutamine, 1mM sodium pyruvate, and 50µM β-mercaptoethanol) at 37 °C and 5% CO₂. Monocytes were differentiated to macrophages in differentiation media (growth media without FBS, supplemented with 1mg/mL BSA and 200nM phorbol-12-myristate-13-acetate (PMA)) within 48–72 hr as evidenced by their adherence to the culture plate.

Kawashima R.L, & Medh J.D. (2014). Down-regulation of lipoprotein lipase increases ABCA1-mediated cholesterol efflux in THP-1 macrophages. Biochemical and biophysical research communications, 450(4), 1416-1421.

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Activation and Expansion of Human NK Cells

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Human NK cells were isolated from peripheral blood of healthy U.S. donors by negative selection (Stemcell Technologies). NK cells were resuspended in Iscove's modified Dulbecco's medium (IMDM; Gibco) supplemented with 10% human serum (Valley Biomedical) and used within 4 days. To obtain IL-2-activated NK cells, freshly isolated NK cells were co-cultured with irradiated autologous feeder cells in OpTimizer (Invitrogen) supplemented with 10% purified IL-2 (Hemagen), 100 units/mL recombinant IL-2 (Roche), and 5 μg/mL phytohemagglutinin (PHA, Sigma) and expanded in the same medium without PHA and feeder cells. The human erythroleukemia cell line K562 (American Type Culture Collection, Manassas, VA) were cultured in RPMI 1640 supplemented with 2 mM L-glutamine and 10% fetal bovine serum (FBS; Atlanta Biologicals).

Zhuang X., Veltri D.P, & Long E.O. (2019). Genome-Wide CRISPR Screen Reveals Cancer Cell Resistance to NK Cells Induced by NK-Derived IFN-γ. Frontiers in Immunology, 10, 2879.

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FBXW7 Mutant Generation and Analysis

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FBXW7 mutants were made by QuikChange Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol. The pTripZ vector (Thermo) was used to generate high-titer Tet-inducible FBXW7 lentivirus. Polyfect (Qiagen) and Calcium Phosphate Transfection Kit (Invitrogen) were used for 293 T transfection and lentivirus production, respectively. MT1 cells were infected with FBXW7 wild-type or mutant virus and maintained in culture with RPMI-1640 with 10% FBS (TET-Tested; Atlanta Biologicals) with puromycin. Induction of FBXW7 was carried out in the presence of doxycycline for various times.

Yeh C.H., Bellon M., Wang F., Zhang H., Fu L, & Nicot C. (2020). Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells. Molecular Cancer, 19, 139.

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Lentiviral transduction of cell lines

Cited 1 time

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BT474, MDA-MB-361, and T47D cells were transduced with an expression cassette encoding Gluc and GFP separated by an internal ribosomal entry site, using a lentiviral vector as previously described (43 (link)). All cell lines, purchased from the American Type Culture Collection, were authenticated (Cell Check). BT474-Gluc and T47D-Gluc cells were maintained in RPMI 1640 supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals). MDA-MB-361-Gluc cells were maintained in Dulbecco’s modified Eagle’s medium/F12 supplemented with 10% (v/v) FBS.

Kodack D.P., Askoxylakis V., Ferraro G.B., Sheng Q., Badeaux M., Goel S., Qi X., Shankaraiah R., Cao Z.A., Ramjiawan R.R., Bezwada D., Patel B., Song Y., Costa C., Naxerova K., Wong C.S., Kloepper J., Das R., Tam A., Tanboon J., Duda D.G., Miller C.R., Siegel M.B., Anders C.K., Sanders M., Estrada M.V., Schlegel R., Arteaga C.L., Brachtel E., Huang A., Fukumura D., Engelman J.A, & Jain R.K. (2017). The brain microenvironment mediates resistance in luminal breast cancer to PI3K inhibition through HER3 activation. Science translational medicine, 9(391), eaal4682.

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