(–)-Cocaine HCl was provided by the National Institute on Drug Abuse Drug Supply Program (Bethesda, MD). d-Amphetamine hemisulfate was purchased from Sigma Aldrich (St. Louis, MO). Bruce Blough (RTI) synthesized lisdexamfetamine mesylate. Cocaine, d-amphetamine, and lisdexamfetamine were dissolved in sterile water, and all solutions were passed through a 0.22 micron Millipore sterile filter (Millipore, Billerica, MA) before use. Drug doses were calculated and expressed using the salt forms listed above.
Millipore sterile filter
Manufactured by Merck Group
Sourced in Germany
The Millipore sterile filter is a laboratory equipment designed for the filtration of liquids. It is a high-performance filter that ensures the effective removal of particulates, microorganisms, and other contaminants from various solutions, providing a sterile filtrate.
Automatically generated - may contain errors
4 protocols using millipore sterile filter
1
Preparation of Psychostimulant Solutions
2
Cocaine, Mecamylamine, and Nicotine Protocol
3
Extraction of Phytochemicals from P. curatellifolia Leaves
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The dried P. curatellifolia leaf powder was weighed using the KERNEG balance (Kern and Son GmbH, D -72336 Balingen, Germany) and the following masses of 10 g, 10 g, 15 g and 15 g were mixed with the solvents methanol, water, acetone and ethanol respectively. A volume of 150 mls of each solvent was added to the leaf powder in a 1000 ml beaker. The beakers were placed on a magnetic stirrer and left stirring for 2 h. The solutions were filtered using Whatman No 1 filter paper (Sigma-Aldrich Chemicals, Steinheim, Germany) or transferred into syringe and filtered into a small glass vial. The sterile suspension was filtered again using 0.45 μM Millipore® sterile filter (Sigma-Aldrich, Taufkirchen, Germany) into a labelled small glass vial. The filtrate was evaporated to dryness, collected and stored at 4 oC until when required.
4
Plant Extract Preparation Protocol
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The preparation of plant extracts was described by Chitemerere and Mukanganyama [12 ]. Briefly, plant samples were ground in a two-speed blender (Cole Parmer Instrument Co., Vernon Hills, USA). A volume of 8 ml of ethanol was added to 2 g of grounded powder and shaken for 5 min on a vortex mixer and left for 10 min. The plant suspension was then transferred into a syringe and filtered into a small glass vial. The sterile suspension was filtered again using 0.45 μM Millipore sterile filter (Sigma-Aldrich, Taufkirchen, Germany) into a labelled small glass vial. Ethanol was left to evaporate overnight in fume hood with an air stream. A constant dry weight of each extract was obtained and the residues were stored at 4°C.
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