Anti mdm2 clone smp14 and clone h 221

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-Mdm2 (clone SMP14 and clone H-221) are mouse monoclonal antibodies that recognize Mdm2, a negative regulator of the tumor suppressor p53. These antibodies can be used to detect and study Mdm2 expression in various applications.

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Lab products found in correlation

Anti lamin b c 20, by Santa Cruz Biotechnology (2 mentions) Anti β actin clone ac 74, by Merck Group (2 mentions) Horseradish peroxidase conjugated secondary antibody, by GE Healthcare (1 mentions) Anti slug clone c19g7, by Cell Signaling Technology (1 mentions) Anti e cadherin clone 24e10, by Cell Signaling Technology (1 mentions) Anti parp1, by Cell Signaling Technology (1 mentions) Anti c myc, by Cell Signaling Technology (1 mentions) Anti slug clone l40c6, by Cell Signaling Technology (1 mentions) Anti e cadherin clone 32a8, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-MDM2 (SMP14, Anti-MDM2 SMP14

2 protocols using anti mdm2 clone smp14 and clone h 221

Immunoblotting and Protein Isolation Protocols

Cited 1 time

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The immunoblotting, isolation of nuclear protein fractions, immunoprecipitation and mice protein extraction procedures were performed as previously described [22 (link)].
The primary antibodies used in this work are as follows: human anti-p53 (clone BP53-12, Novocastra Laboratories, Newcastle Upon Tyne, UK), mouse anti-p53 (clone 1C12, Cell Signaling Technology, Beverly, MA, USA), anti-RPL11 (clone 3A4A7, Invitrogen, Carlsbad, UK), anti-PARP-1 (Cell Signaling Technology), anti-RPS6 (clone C-8, Santa Cruz Biotechnology, CA, USA), anti-RPS14 (clone H-130, Santa Cruz Biotechnology), anti-Mdm2 (clone SMP14 and clone H-221, Santa Cruz Biotechnology), anti-Slug (clone C19G7, Cell Signaling Technology), anti-E-cadherin (clone 24E10, Cell Signaling Technology), anti-c-Myc (clone N-262, Santa Cruz Biotechnology), anti-β-actin (clone AC-74, Sigma-Aldrich), and anti-Lamin B (C-20, Santa Cruz Biotechnology). Horseradish peroxidase-conjugated secondary antibodies were from GE-Healthcare (Milan, Italy).

Brighenti E., Giannone F.A., Fornari F., Onofrillo C., Govoni M., Montanaro L., Treré D, & Derenzini M. (2016). Therapeutic dosages of aspirin counteract the IL-6 induced pro-tumorigenic effects by slowing down the ribosome biogenesis rate. Oncotarget, 7(39), 63226-63241.

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Immunoblotting and Nuclear Protein Fractionation

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The immunoblotting, isolation of nuclear protein fractions and immunoprecipitation procedures were performed as previously described.^{11 (link)} Antibodies used in this work were as follows: anti-p53 (clone BP53-12, Novocastra), anti-β-actin (clone AC-74, Sigma-Aldrich), anti-MDM2 (clone SMP14 and clone H-221, Santa Cruz Biotechnology), anti-L11 (clone 3A4A7, Invitrogen), anti-SLUG (clone L40C6, Cell Signaling Technology), anti-E-cadherin (clone 32A8, Cell Signaling Technology), anti-c-Myc (Cell Signaling Technology) and anti-Lamin B (C-20, Santa Cruz Biotechnology).

Brighenti E., Calabrese C., Liguori G., Giannone F.A., Trerè D., Montanaro L, & Derenzini M. (2014). Interleukin 6 downregulates p53 expression and activity by stimulating ribosome biogenesis: a new pathway connecting inflammation to cancer. Oncogene, 33(35), 4396-4406.

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