Lc msd trap sl mass spectrometer

All reagents were purchased from commercial suppliers and were used without further purification. Melting points (M.p.) were determined on an Electrothermal 9100 melting point apparatus (Weiss-Gallenkamp, Loughborough, UK) and are uncorrected. IR spectra were recorded on an IRPrestige-21 Fourier Transform Infrared spectrophotometer (Shimadzu, Tokyo, Japan). ¹H-NMR and ¹³C-NMR spectra were recorded on a Varian Mercury-400 FT-NMR spectrometer (Agilent, Palo Alto, CA, USA). Mass spectra were recorded on an Agilent LC-MSD-Trap-SL Mass spectrometer (Agilent Technologies, Palo Alto, CA, USA). Elemental analyses were performed on a Perkin Elmer EAL 240 elemental analyzer (Perkin-Elmer, Norwalk, CT, USA) and the results were within ±0.4% of the theoretical values.

All reagents were purchased from commercial suppliers and were used without further purification. Melting points (M.p.) were determined on an Electrothermal 9100 melting point apparatus (Weiss-Gallenkamp, Loughborough, UK) and are uncorrected. IR spectra were recorded on an IRPrestige-21 Fourier Transform Infrared spectrophotometer (Shimadzu, Tokyo, Japan). ¹H-NMR and ¹³C-NMR spectra were recorded on a Varian Mercury-400 FT-NMR spectrometer (Agilent, Palo Alto, CA, USA). Chemical shifts were expressed in parts per million (ppm) and tetramethylsilane was used as an internal standard. Mass spectra were recorded on an Agilent LC-MSD-Trap-SL Mass spectrometer (Agilent Technologies, Palo Alto, CA, USA). Elemental analyses were performed on a Perkin Elmer EAL 240 elemental analyzer (Perkin-Elmer, Norwalk, CT, USA).

General: Solvents were purified by standard methods. All the reagents used were purchased by Sigma-Aldrich and used as received.
TLC analyses were performed using Merck 60 F254 precoated silica gel glass plates. Column chromatography was carried out on Macherey-Nagel silica gel 60 (70–230 mesh).
NMR spectra were recorded using a Bruker AV 500 spectrometer operating at 500 MHz for 1H, 125.8 MHz for 13C. Chemical shifts are reported relative to internal Me4Si. Multiplicity is given as follow: s = singlet, d = doublet, t = triplet, q = quartet, qn = quintet, m = multiplet, br = broad peak.
ESI-MS mass spectra were obtained with an Agilent Technologies LC/MSD Trap SL mass spectrometer. EI/MS spectra were obtained with an Agilent Technologies 6850-5973 GS/MS.