Tryptose phosphate broth

GL261 mouse GBM cells (gift from Dr. G. Sáfrány, National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary) were cultured in Gibco DMEM GlutaMAX (Thermo Fischer Scientific, Waltham, MA, USA) supplemented with 10% Gibco heat-inactivated fetal bovine serum (FBS, Thermo Fischer Scientific), 10 U/mL Gibco penicillin-streptomycin (PEST, Thermo Fisher Scientific), and 1 mM Gibco sodium pyruvate (Thermo Fisher Scientific). Baby hamster kidney (BHK)-21 cells were cultured in Gibco Glasgow’s minimum essential medium (GMEM, Thermo Fisher Scientific) supplemented with 10% FBS, 2% tryptose phosphate broth (Teknova, Hollister, CA, USA), 20 mM HEPES, and 10 U/mL Gibco PEST (Thermo Fisher Scientific).

The murine glioblastoma cell line GL261 (kind gift from Dr. Geza, National Research Institute for Radiobiology and Radiohygiene, Safrany, Hungary) and the murine neuroblastoma cell line NXS2 (kind gift from Dr. Holger N. Lode, University of Greifswald, Germany) were cultured in DMEM Glutamax supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin (PEST), and 1% sodium pyruvate. The murine glioblastoma cell line CT-2A (generously provided by Thomas Seyfried, Boston College) and Firefly-luciferase-expressing CT-2A-Fluc clones (generated by Jan Brun and David Stojdl, Children’s Hospital of Eastern Ontario, Ottawa, Canada) were cultured in RPMI-1640 complemented with 10% FBS, 1% PEST, and 1% sodium pyruvate. BHK21 cells (kind gift from Andres Merits, University of Tartu) were cultured in GMEM (Glasgow’s Modified Eagle Medium) supplemented with 10% FBS, 2% TPB (Tryptose Phosphate Broth, Teknova), 2% 1M HEPES, and 1% PEST. For transducing SFV in BHK21 cells, infectious GMEM medium supplemented with 1% PEST, 2% 1M HEPES, and 10% FBS was used. All cell culture reagents were from Thermo Fisher Scientific (Waltham, MA) if nothing else is stated.