Confocal fluorescence imaging was performed using a Zeiss LSM 710
inverted confocal microscope attached to an EXFO Xcite series 120Q lamp and a
digital Zeiss XM10 camera (Carl Zeiss AG). For LSFM validation experiments, a
transparent section of the heart was placed on a 35 mm glass-bottom petri dish
and mounted in a drop of immersion oil before imaging using 10x and 100x
objectives. The peritoneal adipose tissue was placed over a surface of
~1.6 cm2 and the skeletal muscle was sliced into thin sections
and mounted using ProLong Diamond anti-fade solution (ThermoFisher Scientific).
Tissue slices were exhaustively scanned and the images were taken using 63x
objectives. A Zeiss Zen software was used to process the optical z-stack
sections into a single 2D image.
inverted confocal microscope attached to an EXFO Xcite series 120Q lamp and a
digital Zeiss XM10 camera (Carl Zeiss AG). For LSFM validation experiments, a
transparent section of the heart was placed on a 35 mm glass-bottom petri dish
and mounted in a drop of immersion oil before imaging using 10x and 100x
objectives. The peritoneal adipose tissue was placed over a surface of
~1.6 cm2 and the skeletal muscle was sliced into thin sections
and mounted using ProLong Diamond anti-fade solution (ThermoFisher Scientific).
Tissue slices were exhaustively scanned and the images were taken using 63x
objectives. A Zeiss Zen software was used to process the optical z-stack
sections into a single 2D image.