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N peptide pools

Manufactured by Miltenyi Biotec
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N-peptide pools are collections of synthetic peptides derived from known protein sequences. These peptide pools are designed to facilitate the identification and analysis of immune responses, such as T-cell epitope mapping. The core function of N-peptide pools is to provide a comprehensive set of peptides that can be used as tools for various immunological and biological research applications.

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Lab products found in correlation

Anti cd3, by Thermo Fisher Scientific (2 mentions) Immunospot 4.0 analyzer, by Cellular Technology (2 mentions) Mu ifn γ, by Cellular Technology (2 mentions) Anti ifn γ capture antibody, by Cellular Technology (2 mentions) Sars cov 2 s peptide, by Miltenyi Biotec (2 mentions) Biotin conjugated anti ifn γ, by Cellular Technology (2 mentions) Immunospot software, by Cellular Technology (2 mentions)

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Peptide pools (3 citations)

2 protocols using n peptide pools

1

SARS-CoV-2 Epitope-Specific T-cell Assay

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ELISPOT was performed according to the manufacturer’s instructions (Cellular Technology Ltd.; MU IFN-γ). Plates were coated with anti–IFN-γ capture antibody (Cellular Technology Ltd.) at 4°C overnight. Splenocytes (0.25 × 106) were stimulated in duplicates with SARS-CoV-2 S-peptide (2 μg/ml; Miltenyi Biotec, 130–126-701) or N-peptide pools (2 μg/ml; Miltenyi Biotec, 130–126-699) for 24 hours at 37°C. Splenocytes stimulated with anti-CD3 (1 μg/ml; Thermo Fisher Scientific, 16–0031-82) or medium alone were used as positive and negative control, respectively. This was followed by incubation with biotin-conjugated anti–IFN-γ (Cellular Technology Ltd.) for 2 hours at room temperature and then alkaline phosphatase–conjugated streptavidin for 30 min. The plates were washed and scanned using an ImmunoSpot 4.0 analyzer, and the spots were counted with ImmunoSpot software (Cellular Technology Ltd.) to determine SFC per 106 splenocytes.
Hajnik R.L., Plante J.A., Liang Y., Alameh M.G., Tang J., Bonam S.R., Zhong C., Adam A., Scharton D., Rafael G.H., Liu Y., Hazell N.C., Sun J., Soong L., Shi P.Y., Wang T., Walker D.H., Sun J., Weissman D., Weaver S.C., Plante K.S, & Hu H. (2022). Dual spike and nucleocapsid mRNA vaccination confer protection against SARS-CoV-2 Omicron and Delta variants in preclinical models. Science translational medicine, 14(662), eabq1945.
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2

SARS-CoV-2 Epitope-Specific T-cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISPOT was performed according to the manufacturer’s instructions (Cellular Technology Ltd.; MU IFN-γ). Plates were coated with anti–IFN-γ capture antibody (Cellular Technology Ltd.) at 4°C overnight. Splenocytes (0.25 × 106) were stimulated in duplicates with SARS-CoV-2 S-peptide (2 μg/ml; Miltenyi Biotec, 130–126-701) or N-peptide pools (2 μg/ml; Miltenyi Biotec, 130–126-699) for 24 hours at 37°C. Splenocytes stimulated with anti-CD3 (1 μg/ml; Thermo Fisher Scientific, 16–0031-82) or medium alone were used as positive and negative control, respectively. This was followed by incubation with biotin-conjugated anti–IFN-γ (Cellular Technology Ltd.) for 2 hours at room temperature and then alkaline phosphatase–conjugated streptavidin for 30 min. The plates were washed and scanned using an ImmunoSpot 4.0 analyzer, and the spots were counted with ImmunoSpot software (Cellular Technology Ltd.) to determine SFC per 106 splenocytes.
Hajnik R.L., Plante J.A., Liang Y., Alameh M.G., Tang J., Bonam S.R., Zhong C., Adam A., Scharton D., Rafael G.H., Liu Y., Hazell N.C., Sun J., Soong L., Shi P.Y., Wang T., Walker D.H., Sun J., Weissman D., Weaver S.C., Plante K.S, & Hu H. (2022). Dual spike and nucleocapsid mRNA vaccination confer protection against SARS-CoV-2 Omicron and Delta variants in preclinical models. Science translational medicine, 14(662), eabq1945.
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