Cd127 af488

Manufactured by BioLegend

CD127 AF488 is a fluorescent-labeled antibody that binds to the CD127 (IL-7 receptor alpha) surface antigen. CD127 is expressed on various immune cell types and plays a role in lymphocyte development and homeostasis. The AF488 fluorophore allows for the detection and analysis of CD127-expressing cells using flow cytometry or other fluorescence-based techniques.

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2 protocols using cd127 af488

Phenotyping Treg Subsets in PBMC

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Frozen PBMC from WNV infected subjects were thawed, rested for 30 minutes and then co-stained with antibodies for surface markers and cytokines of interest, including CD4 PerCP (BD, clone RPA-T4), CD25 PE (BioLegend, clone BC96), CD127 AF488 (Biolegend, clone 8019D5), FoxP3 APC (eBioscience, clone PCH101), CTLA-4 PE-CF594 (BD, clone BN13) and CCR4 BV605 (Biolegend, clone L29IH4). After 20 min at 4 degrees, cells were washed and immediately analyzed by flow cytometry.

James E.A., Gates T.J., LaFond R.E., Yamamoto S., Ni C., Mai D., Gersuk V.H., O’Brien K., Nguyen Q.A., Zeitner B., Lanteri M.C., Norris P.J., Chaussabel D., Malhotra U, & Kwok W.W. (2016). Neuroinvasive West Nile Infection Elicits Elevated and Atypically Polarized T Cell Responses That Promote a Pathogenic Outcome. PLoS Pathogens, 12(1), e1005375.

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Detailed Immunological Assays for LB27.4 Cell Line

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The I-A^b+ B cell lymphoma line LB27.4 was purchased from the American Type Culture Collection. The following FACS antibodies were used in this study: Fc Block, CD8a BV 421, CD45.2 BV 510, B220 BV 605, CD127 AF 488, CD45.1 PE, KLRG1 PE-Cy7, CD25 AF 647, TNF-α AF 488, and IFN-γ AF 647 (all from Biolegend). Anti-alpha-adducin (ab40760) from Abcam was used for western blots. Anti-PKC-θ (C-18), anti-alpha-adducin (H-100; used for microscopy), and anti-phospho-alpha-adducin (Ser 726) antibodies were from Santa Cruz Biotechnology. CellTrace Violet, CellTrace CFSE, and AF 568-labeled phalloidin were from ThermoFisher. Class I (257-264) and Class II (323-339) ovalbumin peptides were from AnaSpec. GolgiPlug was from BD Biosciences. Anti-CD3 (145-2C11) and anti-CD28 (37.51) were from Bio-X-Cell.

Thauland T.J., Khan H.A, & Butte M.J. (2019). The Actin-Capping Protein Alpha-Adducin Is Required for T-Cell Costimulation. Frontiers in Immunology, 10, 2706.

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