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Loctite skull adhesive

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Loctite skull adhesive is a specialized lab equipment product designed for adhesive applications. It serves as a bonding agent for various materials. The core function of this product is to provide a strong and durable adhesive solution for specific laboratory needs.

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Lab products found in correlation

Mini pump, by Harvard Apparatus (2 mentions) Vetbond tissue adhesive, by 3M (1 mentions)

4 protocols using loctite skull adhesive

1

Targeted IL-6 Infusion in Mice NAc

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Mice were anesthetized with a mixture of ketamine/xylazine as described in the previous sections and surgically implanted with bilateral NAc guide cannulae (Plastic One). Cannulae (Plastics One) were delivered into the NAc according to bregma coordinates (anteroposterior +1.6 mm, mediolateral +1.5 mm, dorsoventral −4.4 mm) and fixed to the skull with Loctite skull adhesive (Henkel). Saline or a total of 5 ng of IL-6 (diluted at 10 ng/mL in saline solution) was administered over 5 min (0.1 mL /min) using a mini-pump (Harvard Apparatus), followed by a 20-min rest, before subthreshold micro-defeat stress. SI test was conducted 24h later. Mice were then sacrificed and perfused as described above in the IHC section to confirm placement of cannula in the NAc.
Menard C., Pfau M.L., Hodes G.E., Kana V., Wang V.X., Bouchard S., Takahashi A., Flanigan M.E., Aleyasin H., LeClair K.B., Janssen W.G., Labonté B., Parise E.M., Lorsch Z.S., Golden S.A., Heshmati M., Tamminga C., Turecki G., Campbell M., Fayad Z., Tang C.Y., Merad M, & Russo S.J. (2017). Social stress induces neurovascular pathology promoting depression. Nature neuroscience, 20(12), 1752-1760.
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2

Intracerebroventricular Infusion in Mice

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Mice were anesthetized as detailed above. For intracerebroventricular (i.c.v.) infusion, mice were surgically implanted into either left or right (randomly assigned) lateral ventricle with a subcutaneous Alzet minipump (model 1004; Durect-Alzet) and lateral guide cannulae (Brain Infusion Kit 2; Alzet). The following coordinates were used: −0.5 mm anteroposterior, ±1.0 mm mediolateral, −2.0 mm dorsoventral from bregma. One day before surgery, cannulae (28-gauge stainless steel) were filled with JSH-23 (1.0 μM) or vehicle (40% hydroxypropyl β-cyclodextrin/PBS; Wako), and the pedestal within the assembly was affixed by vinyl tubing to a minipump loaded with JSH-23 or vehicle. The minipump was activated to initiate a continued delivery at 0.11 μL/h over 21 d. The surgical procedure began with an incision over the skull, and the skin was spread apart under the scapulae to create an area for positioning the minipump on the back. Cannulae were permanently fixed to the skull with Loctite skull adhesive (Henkel). Cannulae, tubing and minipumps were all secured under the skin using Vetbond tissue adhesive (3M) and two staples.
Ibi D., de la Fuente Revenga M., Kezunovic N., Muguruza C., Saunders J.M., Gaitonde S.A., Moreno J.L., Ijaz M.K., Santosh V., Kozlenkov A., Holloway T., Seto J., García-Bea A., Kurita M., Mosley G.E., Jiang Y., Christoffel D.J., Callado L.F., Russo S.J., Dracheva S., López-Giménez J.F., Ge Y., Escalante C.R., Meana J.J., Akbarian S., Huntley G.W, & González-Maeso J. (2017). Antipsychotic-induced Hdac2 transcription via NF-κB leads to synaptic and cognitive side effects. Nature neuroscience, 20(9), 1247-1259.
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3

Targeted IL-6 Infusion in Mice NAc

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Mice were anesthetized with a mixture of ketamine/xylazine as described in the previous sections and surgically implanted with bilateral NAc guide cannulae (Plastic One). Cannulae (Plastics One) were delivered into the NAc according to bregma coordinates (anteroposterior +1.6 mm, mediolateral +1.5 mm, dorsoventral −4.4 mm) and fixed to the skull with Loctite skull adhesive (Henkel). Saline or a total of 5 ng of IL-6 (diluted at 10 ng/mL in saline solution) was administered over 5 min (0.1 mL /min) using a mini-pump (Harvard Apparatus), followed by a 20-min rest, before subthreshold micro-defeat stress. SI test was conducted 24h later. Mice were then sacrificed and perfused as described above in the IHC section to confirm placement of cannula in the NAc.
Menard C., Pfau M.L., Hodes G.E., Kana V., Wang V.X., Bouchard S., Takahashi A., Flanigan M.E., Aleyasin H., LeClair K.B., Janssen W.G., Labonté B., Parise E.M., Lorsch Z.S., Golden S.A., Heshmati M., Tamminga C., Turecki G., Campbell M., Fayad Z., Tang C.Y., Merad M, & Russo S.J. (2017). Social stress induces neurovascular pathology promoting depression. Nature neuroscience, 20(12), 1752-1760.
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4

D-serine Administration in Hippocampus

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The surgery for D-serine administration was performed 7 days prior to the social interaction test. Mice were placed into a stereotaxic apparatus and bilateral guide cannulae (OD 0.46 mm, Plastics One, Roanoke, VA) were surgically implanted targeting the hippocampus similar to lentivirus infusion; the same coordinates were used and the cannulae were permanently fixed to the skull with Loctite skull adhesive (Henkel, Rocky Hill, CT). D-serine infusion was performed using a microinjection pump 20 min prior to behavioral testing. Twenty-four hours after the last behavioral test, the mice received intra-core Cresyl Fast Violet; 30 min later, they were anesthetized and perfused with phosphate buffered saline (PBS) and 4% paraformaldehyde. Nissl staining of the brain slices was performed using the standard protocol to confirm the site of drug injection.
Wang J., Zhang K., Chen X., Liu X., Teng H., Zhao M, & Sun Z. (2017). Epigenetic Activation of ASCT2 in the Hippocampus Contributes to Depression-Like Behavior by Regulating D-Serine in Mice. Frontiers in Molecular Neuroscience, 10, 139.
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