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Taqman array for cd16

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The TaqMan array for CD16 is a laboratory instrument designed for the detection and quantification of CD16 gene expression. It utilizes real-time PCR technology to provide precise and reliable results. The core function of this product is to enable researchers to analyze the expression levels of the CD16 gene in their samples.

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Lab products found in correlation

Qiaamp dna blood mini kit, by Qiagen (5 mentions) Qx200 droplet reader, by Bio-Rad (4 mentions) Quantasoft v 1, by Bio-Rad (3 mentions) T100 thermal cycler, by Bio-Rad (2 mentions)

5 protocols using taqman array for cd16

1

Determining CD16 Polymorphism in PBMCs

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DNA was extracted from the PBMCs of healthy donors using a QIAamp DNA Blood Mini kit (Qiagen, Valencia, CA), and stored at -80°C until use. The polymorphism of CD16 at amino acid position 158 that is a valine (V) vs. phenylalanine (F) was determined using allele-specific droplet digital polymerase chain reaction (ddPCR) employing the TaqMan array for CD16 (rs396991; Life Technologies, Waltham, MA).23 (link), 32 (link), 33 (link) A master reaction mix was prepared, and 1 μl of genotyping DNA was added. The PCR reaction was performed on a Bio-Rad T100 thermal cycler (Bio-Rad, Hercules, CA) for 40 cycles at 95°C for 10 min, 94°C for 30 sec, and 60°C for 1 min. The plate was read on a Bio-Rad QX200 droplet reader. Data were analyzed with Bio-Rad QuantaSoft v.1.5 software.
Jochems C., Hodge J.W., Fantini M., Tsang K.Y., Vandeveer A.J., Gulley J.L, & Schlom J. (2017). ADCC employing an NK cell line (haNK) expressing the high affinity CD16 allele with avelumab, an anti-PD-L1 antibody. International journal of cancer, 141(3), 583-593.
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2

Genotyping CD16 Polymorphism in PBMCs

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DNA was extracted from PBMCs of four healthy donors using a QIAamp DNA Blood Mini kit (Qiagen, Valencia, CA), and stored at −80°C until use. The polymorphism of CD16 at amino acid position 158 that is a valine vs. phenylalanine was determined using allele-specific droplet digital polymerase chain reaction (ddPCR) employing the TaqMan array for CD16 (rs396991; Life Technologies, Waltham, MA) [31 (link), 33 (link), 34 (link)]. A master reaction mix was prepared, and 1 mL of genotyping DNA was added. The PCR reaction was performed on a Bio-Rad T100 thermal cycler (Bio-Rad, Hercules, CA) for 40 cycles at 95°C for 10 min, 94°C for 30 sec, and 60°C for 1 min. The plate was read on a Bio-Rad QX200 droplet reader. Data were analyzed with Bio-Rad QuantaSoft v.1.5 software.
Jochems C., Hodge J.W., Fantini M., Fujii R., Maurice YM I.I., Greiner J.W., Padget M.R., Tritsch S.R., Tsang K.Y., Campbell K.S., Klingemann H., Boissel L., Rabizadeh S., Soon-Shiong P, & Schlom J. (2016). An NK cell line (haNK) expressing high levels of granzyme and engineered to express the high affinity CD16 allele. Oncotarget, 7(52), 86359-86373.
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3

CD16 polymorphism detection via ddPCR

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DNA was extracted from peripheral blood using the QIAamp DNA Blood Mini kit (Qiagen, CA), and stored at −80°C until use. The polymorphism of CD16 that is a valine (V) versus phenylalanine (F) substitution at amino acid position 158 was determined by performing allele-specific droplet digital polymerase chain reaction (ddPCR) using the TaqMan array for CD16 (rs396991) (Life Technologies, Grand Island, NY) (30 (link)). A master reaction mix was prepared, and 1 μl of genotyping DNA was added. The PCR reaction was performed on a BioRad T100 thermal cycler (BioRad, Hercules, CA) for 40 cycles at 95°C for 10 min, 94°C for 30 s, and 60°C for 1 min. The plate was read on a BioRad QX200 droplet reader. Data were analyzed with BioRad QuantaSoft 1.5.
Boyerinas B., Jochems C., Fantini M., Heery C.R., Gulley J.L., Tsang K.Y, & Schlom J. (2015). Antibody-dependent cellular cytotoxicity (ADCC) activity of a novel anti-PD-L1 antibody avelumab (MSB0010718C) on human tumor cells. Cancer immunology research, 3(10), 1148-1157.
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4

CD16 Polymorphism Analysis in Cancer Patients

Cited 1 time
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To examine the polymorphism of CD16 (valine (V) versus phenylalanine (F) substitution at amino acid position 158), DNA was extracted from PBMC of healthy donors and cancer patients receiving avelumab using the QIAamp DNA Blood Mini kit (Qiagen, Valencia, CA) and stored at −80 °C until use. The FcγRIIIa (CD16) genotype was determined by performing allele-specific droplet digital polymerase chain reaction (ddPCR) using the TaqMan array for CD16 (rs396991; Life Technologies, Carlsbad, CA) [18 (link)]. A master reaction mix was prepared, and 1 μL of DNA was added. The PCR reaction was performed as previously described [3 (link)].
Donahue R.N., Lepone L.M., Grenga I., Jochems C., Fantini M., Madan R.A., Heery C.R., Gulley J.L, & Schlom J. (2017). Analyses of the peripheral immunome following multiple administrations of avelumab, a human IgG1 anti-PD-L1 monoclonal antibody. Journal for Immunotherapy of Cancer, 5, 20.
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5

Genotyping CD16 Polymorphism in PBMCs

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DNA was extracted from PBMCs of healthy donors using a QIAamp DNA Blood Mini Kit (Qiagen, Valencia, CA), and stored at −80°C until use. The polymorphism of CD16 at amino acid position 158 that is a valine (V) vs. phenylalanine (F) was determined using allele-specific droplet digital polymerase chain reaction (PCR) employing the TaqMan array for CD16 (rs396991; Life Technologies, Waltham, MA). 24 (link),26 (link),32 (link) A master reaction mix was prepared, and 1 μL of genotyping DNA was added. The PCR reaction was performed on a Bio-Rad T100 thermal cycler (Bio-Rad, Hercules, CA) for 40 cycles at 95°C for 10 min, 94°C for 30 sec, and 60°C for 1 min. The plate was read on a Bio-Rad QX200 droplet reader. Data were analyzed with Bio-Rad QuantaSoft v.1.5 software.
Fujii R., Schlom J, & Hodge J.W. (2017). A potential therapy for chordoma via antibody-dependent cell-mediated cytotoxicity (ADCC) employing NK or high affinity NK (haNK) cells in combination with cetuximab. Journal of neurosurgery, 128(5), 1419-1427.
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