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Anti h4k20me2 3

Manufactured by Abcam
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Anti-H4K20me2/3 is a laboratory reagent that can be used to detect the presence of di- and trimethylated histone H4 at lysine 20 (H4K20me2/3) in various biological samples. It can be utilized in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

H3663, by Santa Cruz Biotechnology (2 mentions) Sc 56, by Abcam (2 mentions) Anti mcherry, by Thermo Fisher Scientific (2 mentions) M11217, by Merck Group (2 mentions) Alexa fluor conjugated series, by Thermo Fisher Scientific (2 mentions) Lsm 700 multiphoton confocal microscope, by Adobe (2 mentions) Anti h3k27me3, by Merck Group (2 mentions)

2 protocols using anti h4k20me2 3

1

Immunofluorescence Staining of Cellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining was performed using standard procedures7 (link),71 . Primary antibodies used were mouse anti-Fas III (1:200, DSHB, 7G10), anti-HA (1:200; Sigma-Aldrich H3663), anti-PCNA (1:200; Santa Cruz sc-56), anti-GFP (1:1,000; Abcam ab 13970), anti-mKO (1:200; MBL PM051M), anti-mCherry (1:1000; Invitrogen M11217), anti-H3K27me3 (1:200; Millipore 07–449), anti-H4K20me2/3 (1:400; Abcam ab7817), anti-ssDNA (1:100, DSHB) and anti-BrdU (1:200; Abcam ab6326). BrdU analogue was Invitrogen B23151 5-bromo-2´-deoxyuridine (BrdU). Secondary antibodies were the Alexa Fluor-conjugated series (1:1000; Molecular Probes). Confocal images for immunostained fixed sample were taken using Zeiss LSM 700 Multiphoton confocal microscope with 63x or 100x oil immersion objectives and processed using Adobe Photoshop software.
Wooten M., Snedeker J., Nizami Z.F., Yang X., Ranjan R., Urban E., Kim J.M., Gall J., Xiao J, & Chen X. (2019). Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement. Nature structural & molecular biology, 26(8), 732-743.
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2

Immunofluorescence Staining of Cellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining was performed using standard procedures7 (link),71 . Primary antibodies used were mouse anti-Fas III (1:200, DSHB, 7G10), anti-HA (1:200; Sigma-Aldrich H3663), anti-PCNA (1:200; Santa Cruz sc-56), anti-GFP (1:1,000; Abcam ab 13970), anti-mKO (1:200; MBL PM051M), anti-mCherry (1:1000; Invitrogen M11217), anti-H3K27me3 (1:200; Millipore 07–449), anti-H4K20me2/3 (1:400; Abcam ab7817), anti-ssDNA (1:100, DSHB) and anti-BrdU (1:200; Abcam ab6326). BrdU analogue was Invitrogen B23151 5-bromo-2´-deoxyuridine (BrdU). Secondary antibodies were the Alexa Fluor-conjugated series (1:1000; Molecular Probes). Confocal images for immunostained fixed sample were taken using Zeiss LSM 700 Multiphoton confocal microscope with 63x or 100x oil immersion objectives and processed using Adobe Photoshop software.
Wooten M., Snedeker J., Nizami Z.F., Yang X., Ranjan R., Urban E., Kim J.M., Gall J., Xiao J, & Chen X. (2019). Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement. Nature structural & molecular biology, 26(8), 732-743.
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