To culture bacteria from fresh sediment samples, 1 g of the composite sediment was suspended in physiological saline (0.9% NaCl) by vortexing. Serial 10-fold dilutions were cultured on three replicate R2A agar plates to enumerate total bacteria. To enumerate resistant bacteria, serial dilutions were cultured in triplicates on plates supplemented with AZI (15 mg L−1) (Fluka, Germany) for Industry area 1 samples; or sulfamethazine (SMZ; 350 mg L−1) (Sigma, Germany) or oxytetracycline (OTC; 25 mg L−1) (Sigma, Germany) for Industry area 2 samples. Colony forming units (CFU) were counted after a 5 day incubation at 28°C. ARB cultured from sediments from discharge locations were scraped from the plates, pooled and stored in R2A broth containing antibiotic and 15% glycerol at −80°C.
Sulfamethazine smz
Manufactured by Merck Group
Sourced in Germany, United States
Sulfamethazine (SMZ) is a sulfonamide antibiotic compound. It is a white, crystalline powder used in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using sulfamethazine smz
1
Culturing Antibiotic-Resistant Bacteria from Sediments
2
Myoglobin, Sulfamethazine, and Sulfadiazine Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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High-purity standard myoglobin
(95–100%),
sulfamethazine (SMZ), and sulfadiazine (SDZ) were procured from Sigma-Aldrich
Corporation. All of the chemicals and reagents used in this study
were analytical grade and purchased from Sigma-Aldrich. Double distilled
and deionized (Millipore) water was used for the preparation of buffer
for the experiment.
(95–100%),
sulfamethazine (SMZ), and sulfadiazine (SDZ) were procured from Sigma-Aldrich
Corporation. All of the chemicals and reagents used in this study
were analytical grade and purchased from Sigma-Aldrich. Double distilled
and deionized (Millipore) water was used for the preparation of buffer
for the experiment.
3
Characterization of Psychoactive Compounds
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