The activity of LDH in plasma was evaluated with LDH kit (Randox Laboratories, Ltd., Crumlin, UK) according to the manufacturer instructions. The LDH method measures the oxidation of L-lactate to pyruvate with simultaneous reduction of nicotinamide adenine dinucleotide (NAD). The change in absorbance at 340 nm due to the appearance of reduced NAD (NADH) is directly proportional to the LDH activity.
Ldh kit
Manufactured by Randox
Sourced in United Kingdom
The LDH kit is a laboratory reagent used to measure the activity of the enzyme lactate dehydrogenase (LDH) in biological samples. LDH is an important marker for various medical conditions, and the kit provides a reliable and standardized method for its quantification.
Automatically generated - may contain errors
Lab products found in correlation
Creatinine, by Biodiagnostic (1 mentions)
Aspartate aminotransferase, by Biodiagnostic (1 mentions)
Ochratoxin, by Merck Group (1 mentions)
Ck nac uv rate kit, by EKF Diagnostics (1 mentions)
Malondialdehyde, by Biodiagnostic (1 mentions)
Urea, by Biodiagnostic (1 mentions)
Catalase, by Biodiagnostic (1 mentions)
4 protocols using ldh kit
1
Measurement of Plasma LDH Activity
2
Measuring Hepatocyte LDH Release
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Lactate dehydrogenase release in isolated rat hepatocytes was measured as described by Fau et al.[22 (link)]
The cells were centrifuged at 500 × g for 1 min and the supernatant was taken for measuring the LDH activity. The activity was measured by using LDH kit (Randox). About 20 μl from the cell supernatant was added in 180 μl from the mixture of the kit (buffer A + buffer B). The activity is measured spectrophotometrically at 340 nm.
The cells were centrifuged at 500 × g for 1 min and the supernatant was taken for measuring the LDH activity. The activity was measured by using LDH kit (Randox). About 20 μl from the cell supernatant was added in 180 μl from the mixture of the kit (buffer A + buffer B). The activity is measured spectrophotometrically at 340 nm.
3
Ochratoxin-Induced Biochemical Alterations
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All chemicals used in this study were of analytical grade. Ochratoxin was purchased from Sigma Chemical Co. (St. Luis, MO, USA). Asp. Awamori powder was kindly supplied by Prof. K. Hayashi, Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan. The kits of aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein, albumin, urea, creatinine, malondialdehyde (MDA), catalase (CAT), and total antioxidant capacity (TAC) were obtained from Biodiagnostics Co. (Cairo, Egypt). While LDH kit was purchased from Randox Laboratories Ltd. (Crumlin, UK), and CK-MB was obtained from Stanbio™ (CK-NAC [UV-Rate] kit; Boerne, TX, USA).
4
Oxytetracycline and Ascorbic Acid Evaluation
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Oxytetracycline HCl and ascorbic acid were purchased from Adwia Pharmaceuticals, Cairo, Egypt. N. sativa oil (Baraka® 450 mg capsules) was purchased from Pharco pharmaceuticals, Alexandria, Egypt. All kits were purchased from Biodiagnostics Co., Cairo, Egypt. LDH kit was purchased from Randox Laboratories Ltd, U.K. Other chemicals used in this study were analytical grade.
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