Clot lysis was determined by an automated ACL TOP® hemostasis testing system (Instrumentation Laboratory, Bedford, MA, USA), in which the lysing effect is measured as a decrease of turbidity. Briefly, standard (5 concentrations, 400–1200 ng/ml) or multiple dilutions of tenecteplase were pipetted into sample cups (20 μl), thrombin solution (20 μl) was added and then the reaction was started by addition of a freshly mixed plasminogen/fibrinogen solution (200 μl) at 37°C. The measured lysis time was then plotted against tenecteplase concentration on a double-logarithmic scale. Enzyme activity was expressed as % of that of reference standard as assessed within the same experiment.
Acl top hemostasis testing system
Manufactured by Werfen
Sourced in United States
The ACL TOP® hemostasis testing system is a laboratory instrument designed for performing coagulation assays. It offers automated processing of samples and reagents to analyze various parameters related to blood clotting and hemostasis.
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Lab products found in correlation
2 protocols using acl top hemostasis testing system
1
Automated Clot Lysis Assay for Tenecteplase
2
Dabigatran Concentration Measurement Protocol
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DC at the trough and peak times was calculated using HemosIL® direct thrombin inhibitor assay (Instrumentation Laboratory, Bedford, MA, USA). This assay is a dilute thrombin time test, which is based on the reaction between dabigatran and exogenous thrombin added to the diluted patient plasma. The associated clotting time was measured using the ACL TOP hemostasis testing system (Instrumentation Laboratory), and then the concentration of dabigatran was estimated from the reference curve of the known plasma standard of dabigatran using HemosIL® dabigatran calibrators (Instrumentation Laboratory). The trough or peak ΔDC ratio was defined as the trough or peak DC in the period without PPI minus the corresponding DC in the period with PPI divided by the trough or peak DC in the period with PPI. Trough and peak aPTTs were measured using HemosIL® APTT-SP (Instrumentation Laboratory).
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