The fAD-MSCs at passage 2 (P2) were used for identification of cell surface markers. The cells (2.5 × 105 cells) were stained with the following antibodies: conjugated FITC and PE against CD14 (antibody clone TÜK4, MCA1568F; AbD Serotec, UK), CD34 (antibody clone 581, 555821; BD Bioscience, USA), CD44 (antibody clone IM7, ab19622; Abcam, UK), CD45 (antibody clone HI30, 555482; BD Bioscience), CD90 (antibody clone 5E10, 555596; BD Bioscience), and CD105 (antibody clone SN6, MCA1557F; AbD Serotec). The fAD-MSCs for non-specific binding control were stained with the following antibodies: FITC-conjugated mouse IgG1 κ (antibody clone MOPC-21, 555748; BD Bioscience), PE-conjugated mouse IgG1 (antibody clone A85-1, 550083; BD Bioscience), FITC-conjugated mouse IgG2a κ (antibody clone eBM2a, 11-4724-42; eBioscience, USA), and FITC-conjugated rat IgG2b κ (antibody clone A95-1, 553988; BD Bioscience). Cells were analyzed by using a BD FACSCalibur flow cytometer (BD Bioscience). CellQuest Pro software (BD Bioscience) was used for FACS data analysis.
Fitc conjugated mouse igg1 κ
Manufactured by BD
Sourced in United States
FITC-conjugated mouse IgG1 κ is a laboratory reagent that consists of the mouse immunoglobulin G1 kappa (IgG1 κ) class of antibodies conjugated to the fluorescent dye fluorescein isothiocyanate (FITC). This product can be used as a control or reference material in various immunological assays and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Pe conjugated mouse igg1, by BD (1 mentions)
Ebm2a, by Thermo Fisher Scientific (1 mentions)
Cellquest pro software, by BD (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Transcription factor buffer set, by BD (1 mentions)
Facscanto, by BD (1 mentions)
Pe conjugated rat igg2a κ, by BD (1 mentions)
Fitc conjugated anti ifn γ, by BD (1 mentions)
Brefeldin a, by Merck Group (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
3 protocols using fitc conjugated mouse igg1 κ
1
Characterization of Fetal Adipose-Derived Mesenchymal Stem Cells
2
Quantifying Melanocyte Markers
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MITF and Tyrosinase expression levels were determined by flow cytometry. Staining was performed with the BD Transcription Factor Buffer Set, according to the manufacturer’s protocol. The following antibodies were used: PE-conjugated goat anti-mouse Tyrosinase, PE-conjugated goat anti-mouse IgG isotype control, mouse anti-MITF, FITC-conjugated rat anti-mouse IgG1κ, and FITC-conjugated mouse IgG1κ isotype control (BD Biosciences, San Jose, CA, USA). Analyses were carried out using a flow cytometer (FACSCanto, BD Biosciences, San Jose, CA, USA).
3
Cytokine Expression Profiling
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Cells were pre-incubated with PMA (5 ng/ml) and ionomycin (500 ng/ml) for 6 h, and with brefeldin A for 5 h (10 μg/ml; all from Sigma-Aldrich). For cytokine detection, FITC-conjugated anti–IFN-γ (Clone: B27, Cat# 554700, RRID : AB_395517), PE-conjugated anti-IL-5 (Clone: JES1-39D10, Cat# 559332, RRID : AB_397229), or IL-17 (Clone: SCPL1362, Cat# 560436, RRID : AB_1645514), BV711 conjugated anti-IL-13 (Clone: JES10-5A2, Cat# 564288, RRID : AB_2738731), and allophycocyanin-conjugated anti-IL-4 (Clone: 8D4-8, Cat# 560671, RRID : AB_1727546) or IL-21 (Clone: 3A3-N2.1, Cat# 562043, RRID : AB_10896655, all from BD Biosciences) were used alone or together. Isotype control Abs included FITC-conjugated mouse IgG1 κ (Clone: MOPC-21, Cat# 551954, RRID : AB_394297), PE-conjugated rat IgG2a κ (Clone: R35-95, Cat# 559317, RRID : AB_10050484), BV711-conjugated rat IgG1 κ (Clone: R3-34, Cat# 563283, RRID : AB_2869482), and Alexa Fluor (AF)-647-conjugated mouse IgG1 κ (Clone: MOPC-21, Cat# 557732, RRID : AB_396840, all from BD Biosciences).
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