Total RNA from the peri-infarct cortex of each group for three rats (sham, model, and HSHS10.5 groups) was extracted with TRIZol reagent (Life Technologies, USA). Then, an RNeasy mini kit (Qiagen, USA) was used to purify total RNA from infarcted tissue. According to Affymetrix protocol, 250 ng total RNA was used to conduct biotinylated cDNA by Ambion® WT Expression Kit. The cDNA fragments were hybridized using a Clariom D assay (rat, Affymetrix) for 16 h at 45°C. Affymetrix Fluidics Station 450 was used to wash and stain GeneChips. All arrays were scanned using GeneChip® Scanner 3000 7G with Affymetrix® GeneChip Command Console (AGCC).
Clariom d assay rat
Manufactured by Thermo Fisher Scientific
The Clariom D assay (rat) is a comprehensive microarray designed for gene expression analysis in rat samples. It provides a broad coverage of the rat transcriptome, enabling the study of known and novel transcripts.
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Lab products found in correlation
2 protocols using clariom d assay rat
1
Transcriptome analysis of peri-infarct cortex
2
Transcriptomic Analysis of Rat Myocardial Infarction
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Total RNA was extracted from the infarcted tissue using Trizol reagent (Life Technologies, Carlsbad, CA, USA) and puri ed with an RNeasy mini kit (Qiagen, V alencia, CA, USA). Biotinylated cDNA was prepared in line with the standard Affymetrix protocol from 250ng total RNA by using Ambion® WT Expression Kit. Fragmented cDNA was hybridized for 16 h at 45℃ on Clariom D Assay(rat, Affymetrix).
GeneChips were washed and stained in the Affymetrix Fluidics Station 450. All arrays were scanned by Affymetrix® GeneChip Command Console (AGCC) which was installed in GeneChip® Scanner 3000 7G.
GeneChips were washed and stained in the Affymetrix Fluidics Station 450. All arrays were scanned by Affymetrix® GeneChip Command Console (AGCC) which was installed in GeneChip® Scanner 3000 7G.
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