Mouse wg 6 beadchips

Extracted and purified RNA samples from individual samples with a 260/280 > 2.0 and an RNA integrity number >7.0 (Agilent, Wokingham, UK) were deemed acceptable. Following amplification and biotin labelled, mouse RNA was hybridized to Mouse WG-6 BeadChips (Illumina, San Diego, CA, USA) and human RNA to Illumina Human HT12v3.0 BeadChips (Illumina).

Total RNA, including miRNA was extracted using Qiagen miRNeasy kit from 16 whole mouse adrenals taken from animals between 13 and 16 weeks of age, four from each group; male WT (^+/+), male KCNJ5 (^−/−), female WT and female KCNJ5 (^−/−). Quality of extracted RNA was analysed using an Agilent TapeStation (www.genomics.agilent.com), all RNA samples reached RIN numbers >9. The RNAseq analysis was performed by Cambridge Genome Services (www.genomicservices.path.cam.ac.uk) using Ilumina Mouse WG6 beadchips (approximately 45000 markers mapped to NCBI RefSeq build 36.2, release 22) and analysed using the EdgeR Bioconductor package (https://bioconductor.org/packages/release/bioc/html/edgeR.html). Pathway analysis of the differentially expressed genes was performed with the Ingenuity Package Analysis (IPA®) software package (https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis/). The RNAseq data used in this manuscript have been publically deposited on GEO.