Anti hrip3 13526s

Manufactured by Cell Signaling Technology

Anti-hRIP3 (13526S) is a primary antibody that recognizes the human receptor-interacting protein 3 (hRIP3). RIP3 is a serine/threonine protein kinase that plays a role in programmed necrosis, also known as necroptosis. This antibody can be used in various immunological techniques to detect and study the expression and localization of hRIP3 in biological samples.

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Lab products found in correlation

Anti rip1 610459, by BD (1 mentions)

2 protocols using anti hrip3 13526s

Mechanistic Insights into Necroptosis Signaling

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z-VAD and SMAC mimetic compound were used as described previously [24 (link)]. Recombinant TNF and IFN-γ were purified in our lab. Necrostatin-1 (HY-15760) and AP20187 (HY-13992) are bought from MCE. Anti-ICP6 polyclonal antibodies were raised in rabbits using E. coli-expressed His-tagged ICP6 (1–177 aa). The following antibodies were used in this study: anti-Flag (F3165), anti-Myc (SAB4700448), and anti-mRIP3 (R4277) (Sigma); anti-RIP (D94C12), anti-phospho-RIP (65746S), and anti-hRIP3 (13526S) (Cell Signaling Technology); anti-Phospho-RIP3 (ab209384) and anti-FKBP (ab108420) (Abcam); and anti-actin (PM053-7) (MBL).

Hu H., Wu X., Wu G., Nan N., Zhang J., Zhu X., Zhang Y., Shu Z., Liu J., Liu X., Lu J, & Wang H. (2020). RIP3-mediated necroptosis is regulated by inter-filament assembly of RIP homotypic interaction motif. Cell Death and Differentiation, 28(1), 251-266.

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TNF-induced cell death pathway

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The Z-VAD and Smac mimetic were used as described previously (Wang H. et al., 2014 (link)). Recombinant TNF was purified in our laboratory, AP20187 (HY-13992) was purchased from MCE. ICP6 polyclonal antibodies can be obtained by injecting His-ICP6 (1-177 amino acids) expressed in E. coli into rabbits. The following antibodies were used in this study: anti-Flag M2 (F3165), anti-Myc (SAB4700448) (Sigma); anti-RIP1 (610459) (BD Biosciences); anti-RIP1 (D94C12), anti-phospho-RIP1 (65746S) and anti-hRIP3 (13526S) (Cell Signaling Technology); anti-FKBP (ab108420) (Abcam); and anti-actin (PM053-7) (MBL).

Hu H., Wu G., Shu Z., Yu D., Nan N., Yuan F., Liu X, & Wang H. (2020). ICP6 Prevents RIP1 Activation to Hinder Necroptosis Signaling. Frontiers in Cell and Developmental Biology, 8, 595253.

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