The whole body H2O2 concentration in pea aphid was determined as described previously [19 (link) and 67 (link)]. The pea aphids were collected in 50 mM sodium phosphate buffer (pH 7.4) containing 2 mg/mL catalase inhibitor 3-amino-1, 2, 4-trizole (Sigma, St. Louis, MO, USA). After homogenization, the samples were filtered through a 10-kDa molecular weight cutoff spin filter (Millipore, Billeeica, MA, USA). The eluent was collected and the H2O2 concentration was measured with a Hydrogen Peroxide Assay Kit (Invitrogen, Carlsbad, CA, USA) on a fluorescence microplate reader (Tecan, Männedorf, Switzerland) according to the manufacturer’s protocol. The values were normalized to the total amount of protein in the samples. Ten pea aphids from each group were evaluated in the assays.
Hydrogen peroxide assay kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Hydrogen Peroxide Assay Kit is a colorimetric assay designed to quantify hydrogen peroxide concentrations in various sample types. The kit utilizes a proprietary probe that reacts with hydrogen peroxide, producing a colored product that can be measured spectrophotometrically.
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Lab products found in correlation
2 protocols using hydrogen peroxide assay kit
1
Quantifying Pea Aphid Hydrogen Peroxide
2
Measuring H2O2 Production in Cells
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Cells were treated with NE (50 ng/ml, 100 ng/ml, 1 µg/ml or 10 µg/ml) for 2 h, with or without selective inhibitors pretreated for 30 min, and H2O2 production in the cell supernatants was measured by using the Hydrogen Peroxide Assay kit (Invitrogen) according to the manufacturer’s instructions.
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