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Ave 0991

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AVE 0991 is a laboratory product manufactured by MedChemExpress. It is a chemical compound used in research settings. The core function of AVE 0991 is to serve as a research tool for scientific investigations.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) A 779, by Abcam (2 mentions) Z vad fmk, by R&D Systems (1 mentions) Aurintricarboxylic acid ata, by Merck Group (1 mentions) Dmem f12, by Abcam (1 mentions) Ave0991, by Thermo Fisher Scientific (1 mentions) Oligomeric aβ1 42, by Abcam (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Aβ1 42, by Abcam (1 mentions) Dx600, by Phoenix Pharmaceuticals (1 mentions) Angiotensin 1 7, by Merck Group (1 mentions) Horseradish peroxidase labeled goat anti rabbit antibody, by Santa Cruz Biotechnology (1 mentions) Anti immunoglobulin g igg, by Santa Cruz Biotechnology (1 mentions) Fluorescently labeled secondary antibody, by LI COR (1 mentions) Anti β actin, by Proteintech (1 mentions) Anti claudin 5, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Proteintech (1 mentions)

8 protocols using ave 0991

1

Measuring Macrophage Phagocytosis in Diabetic Milieu

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Raw 264.7 cells were obtained from the ATCC (Manassas, VA). The cells were preincubated for 48 hr at 37°C and 5% CO2 under: a. Normal glucose (NG), in DMEM; b. Diabetic milieu (DM) as previously described (29 (link)); c. DM + AII (100 nM); d. DM + AII (Bachem) (100 nM) and A(1-7) (100 nM); e. DM + AII (100 nM) and AVE 0991 (MedChem Express, Princeton, NJ) (10 nM); f. DM + AII (100 nM) and AVE 0991 (100 nM); g. DM + AII (100 nM) and AVE 0991 (1000 nM). Next, the cells were harvested, incubated for 1 h with pHrodo and fixed with 4% formaldehyde solution. Mean fluorescence intensity was determined by flow cytometry using a BD™ LSR II flow cytometer.
Soto M., Gaffney K.J, & Rodgers K.E. (2019). Improving the Innate Immune Response in Diabetes by Modifying the Renin Angiotensin System. Frontiers in Immunology, 10, 2885.
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2

Fluorescent Conjugate-Based Transwell Assay

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Fluorescently labeled rhodamine (tetramethylrhodamine) bovine serum albumin conjugate (Rh-BSA) was purchased from Molecular Probes (Invitrogen). For the apoptosis inhibitors, the caspase inhibitor Z-VAD-FMK (ZVAD) was obtained from R&D systems, and the endonuclease inhibitor aurintricarboxylic acid (ATA) was obtained from Sigma-Aldrich. Corning Costar Transwell permeable support, 6.5mm insert, 0.4μm polycarbonate membrane, 24 well plates were purchased from Sigma-Aldrich. The Mas receptor agonist AVE 0991 from MedChem Express.
Abdul-Hafez A., Mohamed T, & Uhal B.D. (2019). Activation of mas restores hyperoxia-induced loss of lung epithelial barrier function through inhibition of apoptosis. Journal of lung, pulmonary & respiratory research, 6(3), 58-62.
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3

Investigating the Effects of AVE0991 on Alzheimer's Disease

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Mice were injected intraperitoneally with vehicle (0.9% sterile saline) or AVE0991 (a nonpeptide Ang-(1–7) analogue, 10 mg/kg, MedChemExpress LLC, Monmouth Junction, NJ, USA) once a day for 30 consecutive days (see Supplementary Figure S1). The dose and route of AVE0991 administration were chosen based on previous studies.9 (link),19 (link) According to our previous findings, AVE0991 could steadily cross the blood–brain barrier via intraperitoneal injection route.9 (link),19 (link) During the whole experiment, we monitored the general health of mice and did not observe obvious adverse effects or significant changes in their body weight or food intakes. Additionally, systolic blood pressure (SBP) was measured at the beginning and the end of the treatment period using a tail-cuff method as described.19 (link) AVE0991 injection (10 mg/kg/day) did not significantly affect SBP of APP/PS1 mice at the end of the treatment period (106.27±19.09 mmHg vs 102.44±11.67 mmHg, P>0.05).
For cell experiment, AVE0991 was diluted in DMEM/F12 (Gibco, Armonk, NY, USA) to achieve a final concentration at 10 μM. Oligomeric Aβ1–42 (Abcam, Inc., MA, USA) was prepared as described,18 (link),20 (link) and diluted with DMEM/F12 to a final concentration of 5 μM. The dose of AVE0991 and Aβ1-42 in cell experiments was chosen based on previous studies.9 (link),18 (link)
Duan R., Wang S.Y., Wei B., Deng Y., Fu X.X., Gong P.Y., E Y., Sun X.J., Cao H.M., Shi J.Q., Jiang T, & Zhang Y.D. (2021). Angiotensin-(1–7) Analogue AVE0991 Modulates Astrocyte-Mediated Neuroinflammation via lncRNA SNHG14/miR-223-3p/NLRP3 Pathway and Offers Neuroprotection in a Transgenic Mouse Model of Alzheimer's Disease. Journal of Inflammation Research, 14, 7007-7019.
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4

Stimulation of MAS Receptor in Cells

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The MAS receptor agonist AVE0991 was purchased from MedChem Express.
Penicillin streptomycin was purchased from Gibco (ref: 15140-122, Thermo fisher, USA), 0.25 % Trypsin- EDTA was purchased from Gibco (ref: 25200-056,Thermo fisher, USA), 6 well plates were purchased from CellPro, premium labware.
Thiruvenkataramani R.P., Abdul-Hafez A., Gewolb I, & Uhal B. (2020). Mas Receptor Agonist AVE0991 increases surfactant protein expression under hyperoxic conditions in human lung epithelial cells. Journal of lung, pulmonary & respiratory research, 7(4), 85-91.
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5

Stimulation of MAS Receptor in Cells

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The MAS receptor agonist AVE0991 was purchased from MedChem Express.
Penicillin streptomycin was purchased from Gibco (ref: 15140-122, Thermo fisher, USA), 0.25 % Trypsin- EDTA was purchased from Gibco (ref: 25200-056,Thermo fisher, USA), 6 well plates were purchased from CellPro, premium labware.
Thiruvenkataramani R.P., Abdul-Hafez A., Gewolb I, & Uhal B. (2020). Mas Receptor Agonist AVE0991 increases surfactant protein expression under hyperoxic conditions in human lung epithelial cells. Journal of lung, pulmonary & respiratory research, 7(4), 85-91.
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6

Angiotensin Peptide Receptor Agonists and Antagonists

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Angiotensin-(1–7) was purchased from Sigma-Aldrich (St. Louis, MO). Angiotensin-(5–7), angiotensin-(1–2), and angiotensin-(3–4) were from AnaSpec (Fremont, CA), and angiotensin-(1–4) from GenScript (Piscataway, NJ). The MasR agonist AVE0991 was from MedChem Express (Monmouth Junction, NJ) and antagonist A779 from Abcam (Cambridge, MA). The ACE2 inhibitor DX600 was from Phoenix Pharmaceuticals (Burlingame, CA).
Brar G.S., Barrow B.M., Watson M., Griesbach R., Choung E., Welch A., Ruzsicska B., Raleigh D.P, & Zraika S. (2017). Neprilysin Is Required for Angiotensin-(1–7)’s Ability to Enhance Insulin Secretion via Its Proteolytic Activity to Generate Angiotensin-(1–2). Diabetes, 66(8), 2201-2212.
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7

Investigating MAS1 Receptor Agonist and Antagonist

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AVE0991, a nonpeptide agonist of MAS1 receptor [22 (link)], was purchased from Medchem Express Inc. A-779, a selective antagonist of MAS1 receptor, was purchased from Abcam Inc.
Jiang T., Xue L.J., Yang Y., Wang Q.G., Xue X., Ou Z., Gao Q., Shi J.Q., Wu L, & Zhang Y.D. (2018). AVE0991, a nonpeptide analogue of Ang-(1-7), attenuates aging-related neuroinflammation. Aging (Albany NY), 10(4), 645-657.
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8

Measuring Angiotensin Peptide Levels

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All reagents and drugs, unless otherwise stated, were obtained from Abcam (San Diego, CA, USA). AVE 0991 was obtained from MedChem Express (Monmouth Junction, NJ, USA). The anti-cluster of differentiation 11b (CD11b) was obtained from Millipore (Billerica, MA, USA). The anti-zonula occludens-1 (ZO-1), anti-occludin, anti-claudin-5, anti-immunoglobulin G (IgG), and the secondary antibody, horseradish peroxidase-labeled goat anti-rabbit antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-GAPDH and anti-β-actin were from Proteintech Group (Wuhan, China). The fluorescently labeled secondary antibodies were purchased from LI-COR Biosciences (Lincoln, NE, USA). Commercial enzyme-linked immunosorbent assay (ELISA) kits to determine Ang II and Ang-(1–7) levels were obtained from Cusabio Biotech (Wuhan, China).
Mi X., Cao Y., Li Y., Li Y., Hong J., He J., Liang Y., Yang N., Liu T., Han D., Kuang C., Han Y., Zhou Y., Liu Y., Shi C., Guo X, & Li Z. (2021). The Non-peptide Angiotensin-(1–7) Mimic AVE 0991 Attenuates Delayed Neurocognitive Recovery After Laparotomy by Reducing Neuroinflammation and Restoring Blood-Brain Barrier Integrity in Aged Rats. Frontiers in Aging Neuroscience, 13, 624387.
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