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5 protocols using novozym 435 lipase

1

Enzymatic Ring-Opening Polymerization

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Novozym 435 lipase ([9001-62-1], derived from C. antarctica (>5000 U g−1) and immobilized on an acrylic macroporous resin, was kindly donated by Novozymes A/S, Denmark. ε-Caprolactone, δ-valerolactone, PEGMA (300 Da average Mn) were purchased from Fischer Scientific and stored over molecular sieves. HEAA, and 2-MeTHF, were purchased from Sigma Aldrich and used as received. THF and methanol were purchased from Fischer Scientific UK and used without further purification unless otherwise stated. Water was deionised before use.
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2

HPLC Analysis of Heterocyclic Amines

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Cyanidin-3-O-glucose (C3G) with a purity of 98 % was ordered from Durst Biotechnology Co., Ltd (Chengdu, China). HCA standards of IQ, MeIQx, 4,8-DiMeIQx, NorHarman, Harman, PhIP, and AαC were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA). Chromatographic grade of methanol, acetonitrile, ammonium acetate, triethylamine, phosphoric acid, sodium dihydrogen phosphate, disodium hydrogen phosphate and other analytical grade reagents were brought from Sinopharm chemical reagent Co., Ltd (Beijing, China). Methyl cinnamate and 4A molecular sieve of analytical grade were ordered from Maclin Biochemical Technology Co., Ltd (Shanghai, China). Novozym 435 lipase was purchased from Novozymes Biotechnology Co., Ltd (Shenyang, China).
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3

Analytical Quantification of Heterocyclic Amines in Minced Chicken

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Fresh chicken breast was purchased from a local market (Zhanjiang, Guangdong province, China), cleaned, removed the fascia, and minced using a grinder. Cyanidin-3-O-glucose (C3G) with a purity of 98% was ordered from Durst Biotechnology Co., LTD (Chengdu, China). HCA standards of IQ、MeIQ、MeIQx、4,8-DiMeIQxNorHarmanHarman、PhIP、AαC、MeAαC、Trp-P-2、Trp-P-1A were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA). Chromatographic grade of methanol, acetonitrile, ammonium acetate, triethylamine, phosphoric acid, sodium dihydrogen phosphate, disodium hydrogen phosphate and other analytical grade reagents were bought from Sinopharm chemical reagent Co., LTD (Beijing, China). Methyl cinnamate and 4A molecular sieve of analytical grade were ordered from Maclin Biochemical Technology Co., LTD (Shanghai, China). Novozym 435 lipase was purchased from Novozymes Biotechnology Co., LTD (Shenyang, China).
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4

Biocatalytic Synthesis of Phenylglycinol

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Phenylglycinol and capric acid were obtained from Macleans, Shanghai, China. Bio-catalysts of Novozym435 lipase, Lipozyme RM IM, and Lipozyme TL IM were obtained from Novozymes, Beijing, China. Candida sp. 99-125 was purchased from Beijing CTA New Century Biotechnology Co. LTD., Beijing, China, and the lipase was immobilized on diatomaceous earth. The fermentation and immobilization procedure of the lipase were described in our previous research (Tan et al., 2003 (link); Deng et al., 2005 ). All other chemicals used were of analytical grade, and used as obtained.
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5

Lipase-Catalyzed Transesterification of Duck Oil

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Duck oil was offered by Lichen Oil Co., Ltd. (Weifang, China). Novozym 435 lipase (10,000 U/g) was provided by Novozymes Biotechnology Co., Ltd. (Tianjin, China). 2-thiobarbituric acid (≥98.5%) was obtained by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All other chemical reagents were of analytical or guaranteed purity.
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