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Q exactive high field hybrid orbitrap mass spectrometer

Manufactured by Thermo Fisher Scientific
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The Q Exactive High-Field Hybrid Orbitrap mass spectrometer is a high-resolution, high-mass-accuracy mass spectrometer designed for advanced analytical applications. It combines a quadrupole mass filter with an Orbitrap mass analyzer, providing high-quality data for a wide range of samples.

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Lab products found in correlation

Vanquish horizon binary ultrahigh, by Thermo Fisher Scientific (2 mentions) Compound discoverer 3, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Q-exactive high-field hybrid quadrupole orbitrap mass spectrometer Orbitrap Q-Exactive High-Field mass spectrometer Q Exactive hybrid Q–Orbitrap mass spectrometer Q Exactive orbitrap hybrid mass spectrometer Q Exactive High Field (HF) mass spectrometer Q Exactive hybrid mass spectrometer Q Exactive Orbitrap mass spectrometer Q-Exactive Orbitrap mass Exactive Orbitrap mass spectrometer Q Exactive mass spectrometer

2 protocols using q exactive high field hybrid orbitrap mass spectrometer

1

Metabolomic Profiling of Plasma Samples

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Detailed methods are presented in the Online Repository (available at www.jaci-global.org). Briefly, mass spectrometry was performed on plasma samples with a Vanquish Horizon Binary ultrahigh-performance liquid chromatography system coupled with a Q Exactive High-Field Hybrid Orbitrap mass spectrometer (ThermoFisher). Data were analyzed by using Compound Discoverer 3.3 (ThermoFisher). Metabolite retention times were aligned across runs, and sample extracts combined as a global pooled sample were used as quality controls to correct instrumental response draft and evaluate measurement reproducibility (relative SD < 30%). Metabolites were considered high-confidence annotations and retained for analysis if they included accurate mass (5 ppm) match and either (1) retention time (±5%) matching to previously analyzed reference standards, and/or (2) matching to mass spectrometry2 (link) spectra in mzCloud (mzcloud.org) with at least 1 product ion match.
Cottrill K.A., Chandler J.D., Kobara S., Stephenson S.T., Mohammad A.F., Tidwell M., Mason C., Van Dresser M., Patrignani J., Kamaleswaran R., Fitzpatrick A.M, & Grunwell J.R. (2023). Metabolomics identifies disturbances in arginine, phenylalanine, and glycine metabolism as differentiating features of exacerbating atopic asthma in children. The journal of allergy and clinical immunology. Global, 2(3), 100115.
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2

Metabolomic Profiling of Plasma Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Detailed methods are presented in the Online Repository (available at www.jaci-global.org). Briefly, mass spectrometry was performed on plasma samples with a Vanquish Horizon Binary ultrahigh-performance liquid chromatography system coupled with a Q Exactive High-Field Hybrid Orbitrap mass spectrometer (ThermoFisher). Data were analyzed by using Compound Discoverer 3.3 (ThermoFisher). Metabolite retention times were aligned across runs, and sample extracts combined as a global pooled sample were used as quality controls to correct instrumental response draft and evaluate measurement reproducibility (relative SD < 30%). Metabolites were considered high-confidence annotations and retained for analysis if they included accurate mass (5 ppm) match and either (1) retention time (±5%) matching to previously analyzed reference standards, and/or (2) matching to mass spectrometry2 (link) spectra in mzCloud (mzcloud.org) with at least 1 product ion match.
, & Dehmelt H. (1994). Zero-shift tuning in geonium by variation of trapped charge.. Proceedings of the National Academy of Sciences of the United States of America, 91(11), 5043-5045.
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