Ethanolamine ea

Sodium acetate (SA₁₀, 10 mM, pH 5), 2-(N-morpholino)ethanesulfonic acid (MES, 10 mM, pH 5), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES, 10 mM, pH 7.4), phosphate buffer (PBS, 1.4 mM KH₂PO₄, 8 mM Na₂HPO₄, 2.7 mM KCl and 137 mM NaCl, pH 7.4), NaCl, KCl, MgCl₂, ethanolamine (EA) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich, USA, in molecular biology grade or higher. Ethanol for spectroscopy (purity 99.9% or greater) was purchased from Merck, USA. Oligo-ethylene glycol (OEG) thiols terminated with carboxyl group (HS-C₁₁-(EG)₆-OCH₂-COOH) and hydroxyl group (HS-C₁₁-(EG)₄-OH) were purchased from Prochimia, Poland. N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) were purchased from GE Healthcare, USA. Human recombinant 17β-HSD10 and cypD proteins and monoclonal mouse antibody against mitochondrial cypD (anti-cypD) were purchased from Fitzgerald, USA. Aβ 142 fragment was obtained from AnaSpec, USA. All buffers were prepared using deionized water (Q-water, 18 MΩ/cm resistivity, Direct-Q UV3, Millipore, USA), phosphate buffer with high ionic strength (PBS_NaCl) was prepared from PBS by increasing concentration of NaCl to 750 mM, HEPES_BSA was prepared from HEPES by addition of 250 µg/ml BSA, 15 mM KCl and 0.1 mM MgCl₂.

Aluminum nitrate nonahydrate (Al(NO₃)₃·9H₂O) (> 99% purity), 2-amino terephthalic acid (NH₂-H₂BDC ≥ 99%), Choline chloride (> 99% purity), Ethanolamine (EA) (≥ 98% purity), diethanolamine (DEA)(≥ 98% purity), and methyldiethanolamine (MDE) (≥ 99% purity) were purchased from Sigma–Aldrich products. N,N-Dimethylformamide (DMF) (99% purity), Ethanol (> 99% purity) were supplied by Merck. CO₂ gas (> 99.9% purity) was used in gas absorption tests.

Copric chloride dihydrate (ACS), l-/d-cysteine hydrochloride monohydrate (98%), and thioacetamide (98%) were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Trifluoroacetic acid (≥99.9%, HPLC Grade, TFA), isopropyl alcohol (≥99.5%), and β-Mercaptoethanol (99%) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Immunoglobulin G, Albumin, and Transferrin purified from human serum were purchased from Sigma-Aldrich (Darmstadt, Germany). Ethanolamine (EA, ≥ 99.0% ACS), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, ≥ 98.0%), and N-hydroxysuccinimide (NHS, ≥ 98.0%) were purchased from Sigma-Aldrich.