Thirty‐eight Y‐STR loci plus 3 Y‐InDels were co‐amplified using the Yfiler™ Platinum PCR Amplification Kit in a GeneAmp PCR 9700 thermal cycler (Thermo Fisher Scientific) following the manufacturer's instructions. Amplified fragments were detected by capillary electrophoresis on the Applied Biosystems 3500 Genetic Analyzer (Thermo Fisher Scientific). The electrophoresis data were automatically analyzed by GeneMapper® ID‐X software (Thermo Fisher Scientific). Negative control (H2O) and positive control (007) were genotyped in each batch of DNA amplification. We strictly followed the recommendations for the DNA commission of the International Society of Forensic Genetics (ISFG) in the present study (Gusmao et al., 2006 ).
Yfiler platinum pcr amplification kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Yfiler™ Platinum PCR Amplification Kit is a laboratory equipment product designed for the amplification of Y-chromosome short tandem repeat (Y-STR) markers. It provides a sensitive and reliable method for the analysis of Y-chromosome DNA samples.
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2 protocols using yfiler platinum pcr amplification kit
1
Multiplex Y-STR and Y-InDel Genotyping
2
Y-STR Amplification and DNA Quantification
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DNA quantification of the extracts using Qubit™ 1X dsDNA High Sensitivity assay kits was done for the purpose of evaluating the final concentration of DNA. In order to increase the number of amplified Y-STR loci, we used three different kits for the amplification of Y-STRs: Yfiler® Platinum PCR Amplification kit (Thermo Fisher Scientific, Waltham, MA, USA) including 38 Y-STR loci and 3 Y-InDel loci, Goldeneye™ DNA Y Plus PCR Amplification kit (Peoplespot, Beijing, China) including 41 Y-STR loci and 3 Y-InDel loci, and FastDirect DNA 44Y PCR Amplification kit (Biotech Original, Beijing, China) including 44 Y-STRs and 3 Y-InDels, as per the manufacturers’ recommendations. Amplifications were performed in ABI GeneAmp® 9700 PCR System, and all teeth extracts had been processed prior to the amplification of reference sample DNA. To avoid contamination and cross-contamination, PCR preparation was done in the laboratory dedicated particularly to PCR amplification.
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