Adhesion to HT-29 cell was performed following the method described previously (Lee et al. 2015 ) with minor modifications. Human colon cancer cells HT-29 (Hunan Fenghui Bio-technology Co., Ltd.) were maintained in Dulbecco’s Modified Eagle Medium F-12 (DMEM/F12, Gibco, USA), supplemented with 10% (v/v) fetal bovine serum (Zhengjiang Tianhang Biotechnology Co., Ltd.) in a carbon dioxide incubator of 5% CO2 at 37°C for 48 h. The HT-29 cells were harvested and added into the 24-well plate (2 × 106 cells/well), and grown for 48 h. The medium was refreshed daily. A volume of 0.5 ml LAB (1 × 108 CFU/ml) was added into the wells, and the suspension was incubated at 37°C for 2 h. The wells were washed with PBS three times, then the cells were treated with Triton X-100 (BioFRoxx, Germany), and the bacteria were inoculated on the MRS agar plate. The number of the adherent bacterial cells was counted on the plates and the adhesion rate was calculated.
Ht 29
Manufactured by Hunan Fenghui Biotechnology
Sourced in China
The HT-29 is a cell line derived from a human colorectal adenocarcinoma. It is commonly used in cell culture research and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Hct 116, by Hunan Fenghui Biotechnology (3 mentions)
Triton x 100, by neoFroxx (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Selonsertib, by MedChemExpress (1 mentions)
Z devd fmk, by MedChemExpress (1 mentions)
N acetyl l cysteine nac, by MedChemExpress (1 mentions)
Dulbecco modified eagle medium (dmem), by Solarbio (1 mentions)
Sw480, by Hunan Fenghui Biotechnology (1 mentions)
Caco 2, by Hunan Fenghui Biotechnology (1 mentions)
1640 medium, by Meilun (1 mentions)
Penicillin, by Sangon (1 mentions)
Streptomycin, by Sangon (1 mentions)
4 protocols using ht 29
1
Adhesion Assay for HT-29 Colon Cancer Cells
2
Investigating Coxsackievirus B3 Infection in Human Colon Cancer Cell Lines
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The human colon cancer cell line HT-29 was obtained from the State Key Laboratory of Food Science and Technology, Nanchang University (China), and the HCT-116 cell line was purchased from Hunan Fenghui Biotechnology Co., Ltd. (Changsha, China). Both cell lines were cultured in DMEM (Solarbio, Beijing, China) containing 10% fetal bovine serum (FBS, Every Green, Shanghai, China) at 37 °C in a 5% CO2 incubator. CVB3 (Nancy strain; GI: 323432) was propagated in HeLa cells and stored at −80 °C. All cells were infected with CVB3 at a multiplicity of infection (MOI) of 1. CVB3’s replication cycles were inconsistent in HCT116 and HT29 cells, leading us to select different experimental end-points to observe the phenomenon of virus-induced cell death and perform other related experiments. The caspase-3 inhibitor Z-DEVD-FMK (100 μM), ASK1 inhibitor selonsertib (100 μM), and the antioxidant N-acetyl-L-cysteine (NAC) (10 mM or 20 mM) were all purchased from MedChemExpress (Princeton, NJ, USA). Inhibitors were added to cells 2 h before the viruses.
3
Culturing Colorectal Cell Lines
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The human CRC cell lines HT-29, DLD-1, HCT116, SW480, Caco-2 and LOVO, as well as the normal human colorectal cell FHC, were purchased from Hunan Fenghui Biotechnology Co., Ltd. (China). They were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA) containing 10% foetal bovine serum (FBS; HyClone, USA) at 37 °C in a humidified chamber supplemented with 5% CO2.
4
Establishment of 5-Fu-resistant Colorectal Cancer Cells
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Colorectal cancer cell lines HCT-116 and HT-29 were purchased from Fenghui Biotechnology Co., Ltd (Changsha, China) and cultured in 1640 medium (MeilunBio, Dalian, China) plus 10% FBS (Fetal bovine serum, Oricell, Suzhou, China) as well as 1% penicillin (Sangon, Shanghai, China) and streptomycin (Sangon). Cells were cultured at 37°C in a humidified atmosphere of 5% CO2. 5-Fu-resistant HT-29 cells were established by culturing HT-29 cells with 5-Fu (2 μM) for at least three months and then the resistant clones were collected and expanded for a long culture with 100 nM 5-Fu.
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