Dmem medium and fetal bovine serum

Escherichia coli LPS (0111:B4), FITC-conjugated LPS from E. coli 0111:B4 (FITC-LPS) and paraformaldehyde (PFA) was from Sigma-Aldrich (St. Louis, MO). Collagenase Type 4 was from Worthington biochemical Corporation (Lakewood, NJ). Percoll density gradient media was from GE Healthcare (Uppsala, Sweden). Normal goat serum was from Jackson ImmunoResearch (West Grove, PA). Trizol reagent was from Life Technology (Carlsbad, CA). SYBR Green Master and bovine serum albumin (BSA) was from Roche (Basel, Switzerland). DMEM medium and fetal bovine serum (FBS) was from Gibco (Shelton, CT). High-sig ECL western blotting substrate was from Tanon (Shanghai, China). Protein A-Agarose was from Santa Cruz Biotechnology (Dallas, TX). Lactic acid dehydrogenase (LDH) assay kit was from Jiancheng Biotech (Nanjing, China).

GS comes from Jilin Agricultural University, Changchun City, Jilin Province. The GSF was obtained under the following conditions [30 ]: ethanol concentration 75%, liquid-material ratio 20:1, ultrasonic time 60 min, temperature 50° C. After extraction with petroleum ether, the lower layer solution was eluted (70% ethanol) through an AB-8 macroporous resin column. Subsequent concentrated samples were eluted again (70% ethanol) through a polyamide resin column (pH=5 adjusted with glacial acetic acid). The dried sample obtained was GSF and contained about 70% of total flavonoids.
DMEM medium and fetal bovine serum (FBS) (Gibco, Australia); LPS, tetraethylbenzimidazolylcarbocyanine iodide (JC-1), Hoechst 33258 staining kit, Ca²⁺ indicator Fluo-3/AM, SOD, CAT, and MDA detection kit (Beijing Soleibo Technology Co., Ltd.); Cell counting kit-8 (CCK-8, Beijing Labgic Technology Co., Ltd.); Annexin V fitc PI (Shanghai Bioscience Technology Co., Ltd.); 2, 7-dichlorofluorescein diacetate (DCFH-DA) (Beyotime Biotechnology).