The levels of lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) in the culture medium were measured using the Lactate Dehydrogenase Release Kit (Jiancheng Bioengineering Institute, Nanjing, China) and the Creatine Kinase-MB Isoenzyme Assay Kit (Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's instructions.
Creatine kinase mb isoenzyme assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Creatine Kinase-MB Isoenzyme Assay Kit is a laboratory equipment product that measures the concentration of the creatine kinase-MB isoenzyme in a sample. The kit provides the necessary reagents and protocols to quantify this specific enzyme, which is a marker for certain cardiac conditions.
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Lab products found in correlation
Lactate dehydrogenase ldh assay kit, by Nanjing Jiancheng (1 mentions)
Lactate dehydrogenase assay kit, by Nanjing Jiancheng (1 mentions)
Mtt powder, by Merck Group (1 mentions)
11β hsd, by Abcam (1 mentions)
Gapdh primary antibody, by Cell Signaling Technology (1 mentions)
Masson s trichrome stain kit, by Solarbio (1 mentions)
3 protocols using creatine kinase mb isoenzyme assay kit
1
Measurement of LDH and CK-MB Levels
2
Serum Biomarker Analysis in Reperfusion
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Blood was taken 4 h after reperfusion, and the serum was separated by centrifugation at 7,500 rpm for 15 min, and immediately detected or temporarily frozen at −80°C. Serum TnI concentration was measured using an enzyme-linked immunosorbent assay (ELISA) measurement of Troponin I kit (Elabscience, E-EL-M0086c). Serum LDH activity was measured using a Lactate dehydrogenase (LDH) assay kit (Nanjing jiancheng, China). Serum creatine kinase-MB (CK-MB) was measured with a creatine kinase MB isoenzyme Assay Kit (Nanjing jiancheng, China).
3
Molecular Mechanisms of Cardiac Fibrosis
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Deoxycortisol and MTT powder were purchased from Sigma-Aldrich Corporation. Spironolactone tablets were purchased from Hangzhou Minsheng Pharmaceutical Group Co., Ltd. GAPDH primary antibody was purchased from CST (Cell Signaling Technology. Lnc). CTGF and 11β-HSD primary antibodies were purchased from Abcam. All secondary antibodies were purchased from Shanghai Yeasen Biotechnologies Co., Ltd. Creatine kinase MB isoenzyme Assay Kit and Lactate dehydrogenase assay kit were purchased from Nanjing Jiancheng Bioengineering Institute. Masson’s Trichrome Stain Kit was purchased from Beijing Solarbio Science and Technology Co., Ltd.
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