Ab191217

The protein PARP-1 expression immunohistochemistry staining was conducted according to the previous protocol [34 ]. The above SK-OV-3 tumor slides were then incubated with the recombinant anti-PARP-1 antibody (1:200 dilution, ab191217, Abcam).

HUVECs were lysed in ice-cold RIPA lysis buffer (Beyotime, Shanghai, China), and protein concentration was measured by BCA Protein Assay Kit (Beyotime, Shanghai, China). Equal amounts of protein samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes. Next, membranes were blocked with 5% BSA for 1 h at room temperature and then incubated overnight 4°C with the primary antibodies as follows: cathepsin C (Abcam, ab199109, 1:1000), p-p38 MAPK (Abcam, ab178867, 1:1000), p38 MAPK (Abcam, ab170099, 1:5000), p-NF-κB p65 (Abcam, ab76302, 1:1000), NF-κB p65 (Abcam, ab32536, 1:10,000), p-IκBα (Abcam, ab133462, 1:10,000), IκBα (Abcam, ab32518, 1:10,000), Bcl-2 (Abcam, ab196495, 1:2000), Bax (Abcam, ab53154, 1:1000), Birc5 (Abcam, ab76424, 1:5000), Cleaved PARP (Abcam, ab32064, 1:10,000), PARP (Abcam, ab191217, 1:1000) and GAPDH (Abcam, ab9485, 1:2500). On the second day, the membranes were incubated with the corresponding secondary antibody (Abcam, ab205718, 1:50,000) for 1.5 h at room temperature. Protein blots were visualized using electrochemiluminescence (ECL; Beyotime, Shanghai, China) method and analyzed by a Bio-Rad imaging system (Bio-Rad, CA, USA).

Immunoblotting was performed as previously described.^{55 (link)} Briefly, tissues were lysed in lysis buffer and protein concentration was determined by Bradford. Proteins were separated on polyacrylamide gel and transferred to PVDF membrane. The membrane was blocked in 5% milk and subsequently probed with primary antibodies overnight at 4 °C, then incubated with HRP-conjugated secondary antibodies for protein detection. The antibodies used were anti-GFAP (Z0334, Dako, Santa Clara, CA,USA), anti-ALDH1L1 (Ab87117, Abcam, Cambridge, UK), anti-MBP (78896, Cell Signaling Technology, Cambridge, MA, USA), anti-Olig2 (AB9610, Millipore, Billerica, MA, USA), anti-Prrc2a (Sc-78859, Santa Cruz, Dallas, TX, USA), anti-FLAG (F1804, Sigma), anti-Myc (M047-3, MBL, Woburn, MA,USA), anti-Ythdf1 (17479-1-AP, Proteintech Group, Campbell Park, Chicago, IL,USA), anti-Ythdf2 (24744-1-AP, Proteintech Group), anti-PARP1 (ab191217, Abcam), anti-β-actin (60008-1-Ig, Proteintech Group), anti-GAPDH (CW0266A, CWBiotech, Beijing, China), and anti-β-tubulin (CW0098A, CWBiotech). Polyclonal rabbit anti-FTO antibody was affinity-purified from rabbits immunized with 6 × His tagged full-length human FTO protein as previously reported.^{5 (link)} Polyclonal rabbit anti-ALKBH5 antibody was generated against synthesized peptide by CWBio (Beijing) as previously reported.^{4 (link)}