Image software

Manufactured by Media Cybernetics

Sourced in United States

Image software is a digital tool that allows users to view, edit, and manipulate digital images. It provides basic image processing functionalities such as cropping, resizing, and adjusting image properties.

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Lab products found in correlation

7 protocols using image software

Quantifying Splenic Bcl2 Expression

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Five non-overlapping random fields (magnification: 400×; area: 0.071 mm²) of splenic tissue sections were chosen, photographed, and submitted for morphometric study. Quantification of optical color density and the mean area percentage of bcl2 immunohistochemical positive cells in DAB-stained sections was performed using Image software (Media Cybernetics).

Basha E.H., ElShamy A.M., Ibrahim H.A., Safa M.A., Heabah N.A., Awad R., Ismail R., Amer R.M., Salem O.M., Faheem H, & El-Harty Y.M. (2022). The prospective effect of fucoidan on splenic dysfunction caused by oxaliplatin in male rats through endoplasmic stress dynamics. Scientific Reports, 12, 22147.

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Western Blot Analysis of Colorectal Cancer Markers

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CRC tissue samples and cells were washed with ice-cold phosphate-buffered saline (PBS) 3 times and lysed in RIPA buffer supplemented with protease inhibitor. The protein concentration was determined using the bicinchoninic acid method. Equal amounts of proteins (30 µg) were separated by 15% SDS-PAGE and transferred to a PVDF membrane (Immobilon; Millipore Corp., Bedford, MA, USA). After blocking with 5% non-fat milk (diluted with phosphate-buffered saline/Tween-20, TBST) at 37°C for 1 h, the membranes were incubated with primary antibodies: anti-LRH-1 (1:1000, ab41901), anti-matrix metalloproteinase (MMP)2 (1:1,000, ab80737), anti-MMP9 (1:1,000, ab119906), anti-β-catenin (1:500, ab92514), cyclin D1 (1:1,000, ab134175), c-Myc (1:500, ab51156) and GAPDH (1:1,000, ab8245; Abcam, Cambridge, UK) at 4°C overnight. After rinsing with TBST, they were incubated with horseradish peroxidase-conjugated goat anti-mouse or rabbit immunoglobulin G antibodies (1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA). Protein bands were detected using an enhanced chemiluminescence reagent (Pierce Biotechnology, Inc., Rockford, IL, USA). Band intensity was quantified using Image software (Media Cybernetics, Inc., Rockville, MD, USA).

Yan L., Qiu J, & Yao J. (2017). Downregulation of microRNA-30d promotes cell proliferation and invasion by targeting LRH-1 in colorectal carcinoma. International Journal of Molecular Medicine, 39(6), 1371-1380.

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Cerebellar Astrocyte Quantification

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Five non-overlapping random fields (magnification: X400; area: 0.071 mm²) of each cerebellar hemisphere of each animal were chosen, photographed, and submitted for morphometric study. Quantification of optical color density and the mean area percentage of GFAP immunohistochemical positive astrocytes in DAB-stained sections was performed using Image software (Media Cybernetics). The sections for S100 were analyzed using ImageJ Software to count the immunoreactive cells in each cutting.

Amer R.M., Eltokhy A.K., Elesawy R.O., Barakat A.N., Basha E., Eldeeb O.S., Aboalsoud A., Elgharabawy N.M, & Ismail R. (2022). The Ameliorative Effect of Empagliflozin in Vigabatrin-Induced Cerebellar/Neurobehavioral Deficits: Targeting mTOR/AMPK/SIRT-1 Signaling Pathways. Molecules, 27(12), 3659.

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Scratch Wound Healing Assay

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A linear scratch was made using a sterile pipet tip after growing to 90%–100% confluence. Pictures were taken of the same area of the scratch after 0 and 24 h of incubation at 37℃. The remaining area of the wound was judged by Image software (Media Cybernetics, Silver Spring, MD, USA).

Chen S., Sun L., Zhang J., Zhang L, & Liu X. (2022). Oxygenized Low-Density Lipoprotein-Induced ASMC Dysregulation Depends on circ_0000345-Mediated Regulatory Mechanism. Journal of Atherosclerosis and Thrombosis, 29(12), 1849-1863.

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Cytoplasmic p65 Translocation Assay

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Primary bone marrow cells were isolated form MKR mice and seeded on glass coverslips. The cells were incubated in the absence or presence of TNFα (10 ng/mL) in combination with or without PGRN (200 ng/mL) at 4 °C overnight. Following treatment, the medium was removed and cells were fixed using cold acetone/methanol (1:1). Cells were air-dried and rehydrated following fixation. The samples were blocked with 50% goat serum for 30 min at room temperature, and incubated with primary antibody against p65 (1:50 dilution) for 1 h at room temperature. After washing, a secondary goat anti-rabbit IgG antibody conjugated with fluorescein isothiocyanate (Santa Cruz Biotechnology #SC-2012) diluted 1:100 was added for 1 hour at room temperature, and 4,6-diamidino-2-phenylindole (DAPI) was used for staining nuclei. Image software (Media Cybernetics, Rockville, MD, USA) was used to the capture images under Zeiss Axioscope A1 microscope.

Ding Y., Wei J., Hettinghouse A., Li G., Li X., Einhorn T.A, & Liu C.J. (2021). Progranulin Promotes Bone Fracture Healing via TNFR pathways in Mice with Type 2 Diabetes Mellitus. Annals of the New York Academy of Sciences, 1490(1), 77-89.

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Gas Exchange Measurement of Seedlings

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The gas exchanges were measured from 9:00 to 11:00, using an LI-6800 portable photosynthetic system (LI-COR, Lincoln, NE, USA) equipped with a CO₂ control module and a red–blue LED-light source. The photosynthetic-photon-flux density of the leaf chamber was set to 700 μmol m^–2 s^–1. The airflow rate in the leaf chamber was set as 700 μmol s^–1. The concentration of CO₂ in the reference chamber was set at 400 μmol mol^–1 for the measurements. The net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), intercellular CO₂ concentration (Ci), and CO₂ concentration in the leaf chamber (Ca) were simultaneously recorded from seven seedlings per treatment.
The leaf used to measure the gas-exchange parameters were collected, promptly immersed in N_2, and stored at −80 °C. The leaves were positioned with the main veins cut out on A4 paper, and a 4 × 4 cm piece of green cardboard was added as a control. Leaf images were obtained with a digital camera (7D, Canon, Inc., Tokyo, Japan), and image software (Media Cybernetics, Silver Spring, MD, USA) was utilized to measure the leaf area.

Kayoumu M., Iqbal A., Muhammad N., Li X., Li L., Wang X., Gui H., Qi Q., Ruan S., Guo R., Zhang X., Song M, & Dong Q. (2023). Phosphorus Availability Affects the Photosynthesis and Antioxidant System of Contrasting Low-P-Tolerant Cotton Genotypes. Antioxidants, 12(2), 466.

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Western Blot Analysis of Stress Proteins

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Total protein extracted by RIPA lysis buffer (Thermo Scientific) was determined with an enhanced bicinchoninic acid assay kit (CWBio). The protein from each sample was separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (CWBio). The membranes blocked with 5% non‐fat milk for 1 h at room temperature (RT) later were incubated the whole night with primary antibodies targeting HSPA6 (1:2000, ab212044, Abcam), Cyclin B1 (1:3000, ab32053, Abcam), YAP1 (1:5000, ab56701, Abcam), p‐S127‐YAP1 (1:3000, ab226760, Abcam) and β‐actin (1:5000, ab8226, Abcam) at 4°C. The membranes were washed three times with tris‐buffered saline with Tween 60 (TBST) and probed with horseradish peroxidase‐conjugated secondary antibody (1:5000) for 1.5 h at RT. Protein bands were exposed by a chemiluminescence system, and the membranes were visualized by X‐ray films (Bio‐Rad). Densitometric analysis was performed by image software (Media Cybernetics). The relative protein expression levels of proteins measured in this study were normalized to β‐actin.

Zhang L., Zhuo H., Hong Z., Hou J., Cheng J, & Cai J. (2022). HSPA6, a novel prognostic and therapeutic biomarker, associated with Ming classification in gastric cancer. Journal of Clinical Laboratory Analysis, 37(1), e24763.

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