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Agencourt cleanseq magnetic beads

Manufactured by Beckman Coulter
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Agencourt CleanSEQ magnetic beads are a solid phase reversible immobilization (SPRI) technology used for purification of DNA and RNA samples. The beads allow for efficient capture, washing, and elution of nucleic acids, facilitating downstream applications such as sequencing and PCR.

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Lab products found in correlation

Bigdye terminator v3, by Thermo Fisher Scientific (7 mentions) Agencourt ampure xp magnetic beads, by Beckman Coulter (7 mentions) 3130xl genetic analyzer, by Thermo Fisher Scientific (3 mentions) 3130xl genetic analyser, by Thermo Fisher Scientific (3 mentions) L genetic analyser, by Thermo Fisher Scientific (1 mentions) Lithium acetate, by Merck Group (1 mentions) Sodium cyanoborohydride, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions)

Spelling variants of this product

Magnetic beads (19 citations) Agencourt CleanSEQ (15 citations) CleanSEQ (15 citations) Agencourt CleanSEQ beads (4 citations) CleanSeq beads (3 citations) Agencourt CleanSeq® XP magnetic beads (2 citations)

8 protocols using agencourt cleanseq magnetic beads

1

KRAS and BRAF Mutation Detection

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KRAS (exons 2, 3, and 4) and BRAF (exon 15) genes mutational status was assessed by Sanger sequencing, as described elsewhere [37 (link)]. PCR products were purified using Agencourt AMPure XP magnetic beads (Beckman Coulter) and labelled with BigDye® Terminator v3.1 (Applied Biosystems). Agencourt CleanSEQ magnetic beads (Beckman Coulter) were used for post-labeling DNA fragment purification, and sequence analysis was performed on the Applied Biosystems 3130xl Genetic Analyzer.
Amato E., Barbi S., Fassan M., Luchini C., Vicentini C., Brunelli M., Malleo G., Scarpa A, & Malpeli G. (2016). RASSF1 tumor suppressor gene in pancreatic ductal adenocarcinoma: correlation of expression, chromosomal status and epigenetic changes. BMC Cancer, 16, 11.
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2

Genetic Screening for FAP and HNPCC

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All exons of APC and MUTYH for FAP probands and of MLH1, MSH2 and MSH6 for HNPCC probands were analyzed by conventional Sanger sequencing (primer sequences available upon request). PCR products were purified using Agencourt AMPure XP magnetic beads (Beckman Coulter) and labelled with BigDye® Terminator v3.1 (Applied Biosystems). Agencourt CleanSEQ magnetic beads (Beckman Coulter) were used for post-labeling DNA fragment purification, and sequence analysis was performed on the Applied Biosystems 3130xl Genetic Analyzer.
Simbolo M., Mafficini A., Agostini M., Pedrazzani C., Bedin C., Urso E.D., Nitti D., Turri G., Scardoni M., Fassan M, & Scarpa A. (2015). Next-generation sequencing for genetic testing of familial colorectal cancer syndromes. Hereditary Cancer in Clinical Practice, 13, 18.
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3

Targeted Mutational Profiling by Sanger Sequencing

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KRAS (exons 2 and 3)-, GNAS (exons 8 and 9)-, BRAF (exon 15)- and TP53 (exons 5, 6, 7 and 8)-specific PCR fragments were analysed by Sanger sequencing. PCR products were purified using Agencourt AMPure XP magnetic beads (Beckman Coulter), labelled with Big Dye Terminator v 3.1 (Applied Biosystems). Agencourt CleanSEQ magnetic beads (Beckman Coulter) were used for post-labelling purification. Sequence analysis was performed on an Applied Biosystems 3130×l Genetic Analyser.
Amato E., Molin M.D., Mafficini A., Yu J., Malleo G., Rusev B., Fassan M., Antonello D., Sadakari Y., Castelli P., Zamboni G., Maitra A., Salvia R., Hruban R.H., Bassi C., Capelli P., Lawlor R.T., Goggins M, & Scarpa A. (2014). Targeted next-generation sequencing of cancer genes dissects the molecular profiles of intraductal papillary neoplasms of the pancreas. The Journal of Pathology, 233(3), 217-227.
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4

Sanger Sequencing of BRCA1/2 Mutations

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Mutations of BRCA1 and BRCA2 were validated by Sanger sequencing (primer sequences available upon request). PCR products were purified using Agencourt AMPure XP magnetic beads (Beckman Coulter) and labelled with BigDye® Terminator v3.1 (Applied Biosystems). Agencourt CleanSEQ magnetic beads (Beckman Coulter) were used for post-labeling DNA fragment purification, and sequence analysis was performed on the Applied Biosystems 3130xl Genetic Analyzer.
Mafficini A., Simbolo M., Parisi A., Rusev B., Luchini C., Cataldo I., Piazzola E., Sperandio N., Turri G., Franchi M., Tortora G., Bovo C., Lawlor R.T, & Scarpa A. (2016). BRCA somatic and germline mutation detection in paraffin embedded ovarian cancers by next-generation sequencing. Oncotarget, 7(2), 1076-1083.
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5

Validating NGS Results via Sanger Sequencing

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To validate NGS results, KRAS (exons 2, 3), and TP53 (exons 5, 6, 7, 8) specific PCR fragments were analyzed by Sanger sequencing. PCR products were purified using Agencourt AMPure XP magnetic beads (Beckman Coulter), labeled with Big Dye terminator v3.1 (Applied Biosystems). Agencourt CleanSEQ magnetic beads (Beckman Coulter) were used for post-labeling purification. Sequence analysis was performed on an Applied Biosystems 3130xl Genetic Analyser.
Bria E., Pilotto S., Simbolo M., Fassan M., de Manzoni G., Carbognin L., Sperduti I., Brunelli M., Cataldo I., Tomezzoli A., Mafficini A., Turri G., Karachaliou N., Rosell R., Tortora G, & Scarpa A. (2016). Comprehensive molecular portrait using next generation sequencing of resected intestinal-type gastric cancer patients dichotomized according to prognosis. Scientific Reports, 6, 22982.
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6

Sanger Sequencing for Targeted NGS Validation

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To validate NGS results, KRAS (exons 2, 3), EGFR (exons 19, 21), TP53 (exons 5, 6, 7, 8), and CTNNB1, PIK3CA, SMAD4 and MET specific PCR fragments were analyzed by Sanger sequencing. PCR products were purified using Agencourt AMPure XP magnetic beads (Beckman Coulter), labeled with Big Dye terminator v3.1 (Applied Biosystems). Agencourt CleanSEQ magnetic beads (Beckman Coulter) were used for post-labeling purification. Sequence analysis was performed on an Applied Biosystems 3130xl Genetic Analyser.
Bria E., Pilotto S., Amato E., Fassan M., Novello S., Peretti U., Vavalà T., Kinspergher S., Righi L., Santo A., Brunelli M., Corbo V., Giglioli E., Sperduti I., Milella M., Chilosi M., Scarpa A, & Tortora G. (2015). Molecular heterogeneity assessment by next-generation sequencing and response to gefitinib of EGFR mutant advanced lung adenocarcinoma. Oncotarget, 6(14), 12783-12795.
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7

Genetic Mutation Profiling by Sanger Sequencing

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KRAS (exons 2, 3), GNAS (exons 8, 9), BRAF (exon 15) and TP53 (exons 5, 6, 7, 8), specific PCR fragments were analysed by Sanger sequencing. PCR products were purified using Agencourt AMPure XP magnetic beads (Beckman Coulter), labeled with Big Dye Terminator v3.1 (Applied Biosystems). Agencourt CleanSEQ magnetic beads (Beckman Coulter) were used for post-labeling purification., Sequence analysis was performed on an Applied Biosystems 3130xl Genetic Analyser.
Amato E., Molin M.D., Mafficini A., Yu J., Malleo G., Rusev B., Fassan M., Antonello D., Sadakari Y., Castelli P., Zamboni G., Maitra A., Salvia R., Hruban R.H., Bassi C., Capelli P., Lawlor R.T., Goggins M, & Scarpa A. (2014). Targeted next-generation sequencing of cancer genes dissects the molecular profiles of intraductal papillary neoplasms of the pancreas. The Journal of Pathology, 233(3), 217-227.
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8

Glycoprotein Analysis of Therapeutic Antibodies

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Water (HPLC grade), acetonitrile, immunoglobulin G, lithium acetate, acetic acid, and sodium-cyanoborohydride were obtained from Sigma-Aldrich (St. Louis, MO, USA). APTS and carbohydrate separation buffer (NCHO) were from SCIEX (Brea, CA, USA). The Agencourt CleanSeq magnetic beads were from Beckman Coulter (Brea, CA, USA), and the PNGase F enzyme and digestion reaction kit was from ProZyme (Hayward, CA, USA). The murine mAb standard was from Waters (Milford, MA, USA). The therapeutic antibody samples [innovator, biosimilar, and antibody drug conjugate (ADC)] were the kind gift of HLBS International (Seattle, WA, USA).
Szigeti M., Lew C., Roby K, & Guttman A. (2016). Fully Automated Sample Preparation for Ultrafast N-Glycosylation Analysis of Antibody Therapeutics. Journal of laboratory automation, 21(2).
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