We imaged cells incubated underneath a layer of solid LB-1.5% agarose (1–2 mm). 1 μL of cell culture was placed between a slab LB-agarose and a glass cover slip-bottomed 50 mm petri dish with 30 mm glass diameter (MatTek Corporation). Images were acquired using an inverted fluorescence microscope (Nikon) and imaged every 3 min.
Glass cover slip bottomed 50 mm petri dish with 30 mm glass diameter
Manufactured by MatTek
The Glass cover slip-bottomed 50 mm petri dish with 30 mm glass diameter is a laboratory equipment product. It provides a clear glass surface for cell culture or microscopy applications.
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2 protocols using glass cover slip bottomed 50 mm petri dish with 30 mm glass diameter
1
Live-cell Imaging Under Agarose Gel
2
Live-cell Imaging Under Agarose Gel
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We imaged cells incubated underneath a layer of solid LB-1.5% agarose (1–2 mm). 1 μL of cell culture was placed between a slab LB-agarose and a glass cover slip-bottomed 50 mm petri dish with 30 mm glass diameter (MatTek Corporation). Images were acquired using an inverted fluorescence microscope (Nikon) and imaged every 3 min.
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