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Cd83 bv421

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CD83-BV421 is a fluorochrome-conjugated antibody that specifically binds to the CD83 antigen, which is a cell surface protein expressed on mature dendritic cells. This product can be used for the identification and analysis of CD83-positive cells in flow cytometry applications.

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Lab products found in correlation

Cd11c pe cy7, by BD (2 mentions) Lsrii flow cytometer, by BD (1 mentions) Cd80 fitc, by BD (1 mentions) Hla dr v500, by BD (1 mentions) Cd11c bv605, by BioLegend (1 mentions) Primeflow rna assay, by Thermo Fisher Scientific (1 mentions) Facsverse flow cytometer, by BD (1 mentions) Facsuite software, by BD (1 mentions)

2 protocols using cd83 bv421

1

Multicolor Flow Cytometry for Dendritic Cells

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For flow analysis, 5 × 105 cells were stained in 100 μl staining buffer (PBS, 1% BSA) for 30 minutes at 4°C. Events were acquired on an LSRII flow cytometer (BD Bioscience, San Jose, CA) and analysis was performed in FlowJo 9.5.2 (Treestar Inc., Ashland, OR). Antibodies used for flow cytometric analysis were: CD80-FITC (BD), CD83-BV421 (BioLegend, San Diego, CA), HLA-DR-V500 (BD), CD11c-PECy7 (BD), CD11c-BV605 (BioLegend), PD-L1-FITC (clone M1H1, BD), B7-DC (PD-L2)-PerCPEFluor710 (eBioscience, San Diego, CA) and IDO-PE (clone 700838) (R&D Systems, Minneapolis, MN).
Jochems C., Fantini M., Fernando R.I., Kwilas A.R., Donahue R.N., Lepone L.M., Grenga I., Kim Y.S., Brechbiel M.W., Gulley J.L., Madan R.A., Heery C.R., Hodge J.W., Newton R., Schlom J, & Tsang K.Y. (2016). The IDO1 selective inhibitor epacadostat enhances dendritic cell immunogenicity and lytic ability of tumor antigen-specific T cells. Oncotarget, 7(25), 37762-37772.
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2

Evaluating IDO1 mRNA Expression in DCs

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The expression of IDO1 mRNA was evaluated by the human PrimeFlow™ RNA assay (eBioscience) per the manufacturer's instructions. Briefly, after surface staining of mature or immature DCs using CD11c-PE-Cy7 (BD) and CD83-BV421 (BioLegend), cells were permeabilized, and then hybridized with the type 4 Human RPL13A Alexa Fluor® 488 (Affymetrix, cat. VA4-13187) and the type 1 IDO 1 Human Alexa Fluor® 647 (Affymetrix, cat. VA1-14451) probes, per the protocol instructions. After hybridization, cells were acquired using the BD FACSVerse™ flow cytometer (BD). Analysis to detect IDO mRNA transcripts was performed using the BD FACSuite™ software (BD).
Jochems C., Fantini M., Fernando R.I., Kwilas A.R., Donahue R.N., Lepone L.M., Grenga I., Kim Y.S., Brechbiel M.W., Gulley J.L., Madan R.A., Heery C.R., Hodge J.W., Newton R., Schlom J, & Tsang K.Y. (2016). The IDO1 selective inhibitor epacadostat enhances dendritic cell immunogenicity and lytic ability of tumor antigen-specific T cells. Oncotarget, 7(25), 37762-37772.
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