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Line 1 orf1p

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LINE-1 ORF1p is a protein encoded by the open reading frame 1 (ORF1) of the LINE-1 retrotransposon in the human genome. It is involved in the mobilization and regulation of the LINE-1 element.

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Lab products found in correlation

Mphosph8, by Proteintech (2 mentions) Pphln1, by Merck Group (2 mentions) Morc2, by Fortis Life Sciences (2 mentions) Setdb1, by Proteintech (2 mentions) Dcaf1, by Proteintech (2 mentions) Flag tag (flag), by Novus Biologicals (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Pchk2 t68, by Cell Signaling Technology (2 mentions) P p65 s536, by Cell Signaling Technology (2 mentions) P p53 s15, by Cell Signaling Technology (2 mentions) Abf124, by Thermo Fisher Scientific (2 mentions) Sting, by Thermo Fisher Scientific (2 mentions) γh2ax, by Merck Group (2 mentions)

4 protocols using line 1 orf1p

1

Western Blot Analysis of Protein Markers

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Cells were washed in PBS, counted, normalized for cell number, and lysed directly in 1x SDS-PAGE sample buffer. Samples were run on NuPage 4–12% Bis-Tris gels followed by blotting onto nitrocellulose membranes. Primary antibodies used: FAM208A (Atlas, HPA00875), MPHOSPH8 (Proteintech, 16796–1-AP), PPHLN1 (Sigma, HPA038902), SETDB1 (Proteintech 11231–1-AP), DCAF1 (Proteintech, 11612–1-AP), FLAG (Novus, NB600–345), FLAG (Sigma, F1804, used for IP), MORC2 (Bethyl Labs, A300–149A), LINE-1 ORF1p (Millipore, MABC1152),and HA (Biolegend, 901501).
Yurkovetskiy L., Guney M.H., Kim K., Goh S.L., McCauley S., Dauphin A., Diehl W, & Luban J. (2018). Primate immunodeficiency virus Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex. Nature microbiology, 3(12), 1354-1361.
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2

Western Blot Analysis of Protein Markers

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Cells were washed in PBS, counted, normalized for cell number, and lysed directly in 1x SDS-PAGE sample buffer. Samples were run on NuPage 4–12% Bis-Tris gels followed by blotting onto nitrocellulose membranes. Primary antibodies used: FAM208A (Atlas, HPA00875), MPHOSPH8 (Proteintech, 16796–1-AP), PPHLN1 (Sigma, HPA038902), SETDB1 (Proteintech 11231–1-AP), DCAF1 (Proteintech, 11612–1-AP), FLAG (Novus, NB600–345), FLAG (Sigma, F1804, used for IP), MORC2 (Bethyl Labs, A300–149A), LINE-1 ORF1p (Millipore, MABC1152),and HA (Biolegend, 901501).
Yurkovetskiy L., Guney M.H., Kim K., Goh S.L., McCauley S., Dauphin A., Diehl W, & Luban J. (2018). Primate immunodeficiency virus Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex. Nature microbiology, 3(12), 1354-1361.
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3

Antibody-based Detection of DNA Damage Signaling

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The following antibodies were used: DNMT1 (Abcam ab13537; 1:1000), cGAS (Millipore ABF124; 1:1000 for western blot), STING (Thermo Fisher Scientific PA5–70420; 1:1000 for western blot), MX1 (Thermo Fisher Scientific PA5–22149; 1:1000 for western blot), p65 (Cell Signaling Technology 8242; 1:1000 for western blot), p-p65 S536 (Cell Signaling Technology 3033; 1:1000 for western blot), Chk2 (Cell Signaling Technology 2662; 1:1000 for western blot), p-Chk2 T68 (Cell Signaling Technology 2661; 1:1000 for western blot), p53 (Cell Signaling Technology 2524: 1:500 for western blot), p-p53 S15 (Cell Signaling Technology 12571: 1:500 for western blot), S9.6 (Kerafast ENH001: 1:500 for immunofluorescence), J2 (Kerafast ES2001: 1:60 for immunofluorescence), LINE-1 ORF1p (Millipore MABC1152; 1:1000 for western blot), γH2AX (Millipore 05–636-I; 1:1000 for western blot).
Zhao Y., Oreskovic E., Zhang Q., Lu Q., Gilman A., Lin Y.S., He J., Zheng Z., Lu J.Y., Lee J., Ke Z., Ablaeva J., Sweet M., Horvath S., Zhang Z., Nevo E., Seluanov A, & Gorbunova V. (2021). Transposon-triggered innate immune response confers cancer resistance to the blind mole rat. Nature immunology, 22(10), 1219-1230.
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4

Antibody-based Detection of DNA Damage Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: DNMT1 (Abcam ab13537; 1:1000), cGAS (Millipore ABF124; 1:1000 for western blot), STING (Thermo Fisher Scientific PA5–70420; 1:1000 for western blot), MX1 (Thermo Fisher Scientific PA5–22149; 1:1000 for western blot), p65 (Cell Signaling Technology 8242; 1:1000 for western blot), p-p65 S536 (Cell Signaling Technology 3033; 1:1000 for western blot), Chk2 (Cell Signaling Technology 2662; 1:1000 for western blot), p-Chk2 T68 (Cell Signaling Technology 2661; 1:1000 for western blot), p53 (Cell Signaling Technology 2524: 1:500 for western blot), p-p53 S15 (Cell Signaling Technology 12571: 1:500 for western blot), S9.6 (Kerafast ENH001: 1:500 for immunofluorescence), J2 (Kerafast ES2001: 1:60 for immunofluorescence), LINE-1 ORF1p (Millipore MABC1152; 1:1000 for western blot), γH2AX (Millipore 05–636-I; 1:1000 for western blot).
Zhao Y., Oreskovic E., Zhang Q., Lu Q., Gilman A., Lin Y.S., He J., Zheng Z., Lu J.Y., Lee J., Ke Z., Ablaeva J., Sweet M., Horvath S., Zhang Z., Nevo E., Seluanov A, & Gorbunova V. (2021). Transposon-triggered innate immune response confers cancer resistance to the blind mole rat. Nature immunology, 22(10), 1219-1230.
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