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Phospho stat1 alexafluor 647

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Phospho-STAT1-AlexaFluor 647 is a fluorescently-labeled antibody that specifically detects the phosphorylated form of the Signal Transducer and Activator of Transcription 1 (STAT1) protein. This antibody is conjugated to the AlexaFluor 647 fluorophore, enabling detection and quantification of phosphorylated STAT1 using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Anti il4 blocking antibody clone 11b11, by Thermo Fisher Scientific (2 mentions) Anti h3k27ac ab4729 antibody, by Abcam (2 mentions) Phospo stat1, by Merck Group (2 mentions) Stat6, by Merck Group (2 mentions) Phospho stat6, by Merck Group (2 mentions) Phospho stat6 pe, by BD (2 mentions) Alexa fluor 488 goat anti rabbit secondary antibody, by Thermo Fisher Scientific (2 mentions) Recombinant mouse ifn γ and il 4, by R&D Systems (2 mentions) Stat1, by Merck Group (2 mentions) Phospho erk1 2, by Merck Group (2 mentions) Phospho akt, by Merck Group (2 mentions) Anti actin a4700, by Merck Group (2 mentions)

2 protocols using phospho stat1 alexafluor 647

1

Signaling Mechanisms in Macrophage Activation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant mouse IFN-γ and IL-4 were from R&D Systems. Unless otherwise stated, cytokines were used at a concentration of 100ng/ml and 10ng/ml, respectively. Anti-IL4 blocking antibody (clone 11B11) was from eBioscience. CD11B (clone M1/70), F4/80 (BM8), NOS2 (CXNFT) antibodies for flow cytometry were from eBioscience. Antibodies used in ChIP experiments against Stat1 (sc-592), Stat6 (sc-981), MYC (sc-764), JUNB (sc-46x), C/EBPβ (sc-150X) were all from Santa Cruz. The anti-H3K27Ac (ab4729) antibody was from Abcam. The anti-Pu.1 rabbit polyclonal antibody was generated in-house against the N-terminus of Pu.1 (aa. 1-100; NP_035485.1) 32 (link). Antibodies used in western blot experiments: Stat1 (#9172), phospo-Stat1 (Tyr701, #9171), Stat6 (#9362), phospho-Stat6 (Tyr641, #9361), Erk1/2 (#9102), phospho-Erk1/2 (Thr202/Tyr204, #9101), phospho-Akt (Ser473, #9271); anti-actin (A4700) from Sigma-Aldrich. The following antibodies were used for flow cytometry: CD11b (clone M1/70), F4/80 (BM8), Nos2 (CXNFT) from eBioscience; phospho-Stat1-AlexaFluor 647 (Cat. 612597) and phospho-Stat6-PE (Cat. 558252) from BD Biosciences. The following antibodies were used for immunofluorescence: Alexafluor 488 goat anti-rabbit secondary antibody (Cat. R37116) and DAPI (Cat. 62247) from Thermo Scientific; phospho-Stat1-AlexaFluor 647 (Cat. 612597) and phospho-Stat6-PE (Cat. 558252) from BD Biosciences.
Piccolo V., Curina A., Genua M., Ghisletti S., Simonatto M., Sabò A., Amati B., Ostuni R, & Natoli G. (2017). Opposing macrophage polarization programs show extensive epigenomic and transcriptional cross talks. Nature immunology, 18(5), 530-540.
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2

Signaling Mechanisms in Macrophage Activation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant mouse IFN-γ and IL-4 were from R&D Systems. Unless otherwise stated, cytokines were used at a concentration of 100ng/ml and 10ng/ml, respectively. Anti-IL4 blocking antibody (clone 11B11) was from eBioscience. CD11B (clone M1/70), F4/80 (BM8), NOS2 (CXNFT) antibodies for flow cytometry were from eBioscience. Antibodies used in ChIP experiments against Stat1 (sc-592), Stat6 (sc-981), MYC (sc-764), JUNB (sc-46x), C/EBPβ (sc-150X) were all from Santa Cruz. The anti-H3K27Ac (ab4729) antibody was from Abcam. The anti-Pu.1 rabbit polyclonal antibody was generated in-house against the N-terminus of Pu.1 (aa. 1-100; NP_035485.1) 32 (link). Antibodies used in western blot experiments: Stat1 (#9172), phospo-Stat1 (Tyr701, #9171), Stat6 (#9362), phospho-Stat6 (Tyr641, #9361), Erk1/2 (#9102), phospho-Erk1/2 (Thr202/Tyr204, #9101), phospho-Akt (Ser473, #9271); anti-actin (A4700) from Sigma-Aldrich. The following antibodies were used for flow cytometry: CD11b (clone M1/70), F4/80 (BM8), Nos2 (CXNFT) from eBioscience; phospho-Stat1-AlexaFluor 647 (Cat. 612597) and phospho-Stat6-PE (Cat. 558252) from BD Biosciences. The following antibodies were used for immunofluorescence: Alexafluor 488 goat anti-rabbit secondary antibody (Cat. R37116) and DAPI (Cat. 62247) from Thermo Scientific; phospho-Stat1-AlexaFluor 647 (Cat. 612597) and phospho-Stat6-PE (Cat. 558252) from BD Biosciences.
Piccolo V., Curina A., Genua M., Ghisletti S., Simonatto M., Sabò A., Amati B., Ostuni R, & Natoli G. (2017). Opposing macrophage polarization programs show extensive epigenomic and transcriptional cross talks. Nature immunology, 18(5), 530-540.
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