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Anti cd14 pecy7

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Anti-CD14-PECy7 is a fluorescent-conjugated monoclonal antibody that binds to the CD14 cell surface antigen. CD14 is expressed on monocytes, macrophages, and neutrophils. The PECy7 fluorescent label allows for the detection and analysis of CD14-positive cells using flow cytometry.

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Lab products found in correlation

Anti cd19 apc, by BD (2 mentions) Anti cd3 pe, by BD (2 mentions) Anti cd38 apc, by BD (2 mentions) Anti cd38 fitc, by BD (2 mentions) Facscalibur cell analyzer, by BD (2 mentions) Anti cd5 fitc, by BD (2 mentions) Permeabilization buffer, by Thermo Fisher Scientific (1 mentions) Ab182032, by Abcam (1 mentions) Ab22048, by Abcam (1 mentions) Ab32618, by Abcam (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Sc 459, by Santa Cruz Biotechnology (1 mentions) Anti tlr4 pe, by BD (1 mentions)

3 protocols using anti cd14 pecy7

1

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed with ice-cold PBS once, incubated with appropriate
fluorochrome-conjugated antibodies for 30 min at 4°C and
washed twice with ice-cold PBS containing 0.5% FCS. The following antibodies
were used: anti-CD3-PE (mouse, BD Biosciences, 555333), anti-CD5-FITC (mouse, BD
Biosciences, 555352), anti-CD14-PECy7 (mouse, ebioscience, 25–0149-42),
anti-CD19-APC (mouse, BD Biosciences, 555415), anti-CD27-PE (mouse BD
Biosciences, 555441), anti-CD38-APC (mouse, BD Biosciences, 555462),
anti-CD38-FITC (mouse, BD Biosciences, 555459). Surface protein expression was
detected by a BD FACSCalibur cell analyzer (BD Biosciences) and data were
analyzed using the FlowJo software.
Lee S.H., Singh I., Tisdale S., Abdel-Wahab O., Leslie C.S, & Mayr C. (2018). Widespread intronic polyadenylation inactivates tumor suppressor genes in leukemia. Nature, 561(7721), 127-131.
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2

Lipopolysaccharide-Induced Signaling Pathway Analysis

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Lipopolysaccharides (0111:B4) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Antibodies specific for GST (sc-459) and β-actin (sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies specific for GRP78 (ab32618), CD14 (ab182032), and TLR4 (ab22048) were obtained from Abcam (Cambridge, UK). Antibodies specific for interferon regulatory transcription factor (IRF)3 (4302) and IRF3 phosphorylated at Ser396(4947) were obtained from Cell Signaling Technology (Danvers, MA, USA). F(ab′)2-goat anti-mouse Alexa Fluor®555 (AF555) was purchased from Thermo Fisher Scientific. Anti-TLR4-PE was obtained from PharMingen (Becton Dickinson, Franklin Lakes, NJ, USA). Anti-LAMP1-PE, anti-CD14-PECy7, and permeabilization buffer were obtained from eBioscience (San Diego, CA, USA).
Qin K., Ma S., Li H., Wu M., Sun Y., Fu M., Guo Z., Zhu H., Gong F., Lei P, & Shen G. (2017). GRP78 Impairs Production of Lipopolysaccharide-Induced Cytokines by Interaction with CD14. Frontiers in Immunology, 8, 579.
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3

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed with ice-cold PBS once, incubated with appropriate
fluorochrome-conjugated antibodies for 30 min at 4°C and
washed twice with ice-cold PBS containing 0.5% FCS. The following antibodies
were used: anti-CD3-PE (mouse, BD Biosciences, 555333), anti-CD5-FITC (mouse, BD
Biosciences, 555352), anti-CD14-PECy7 (mouse, ebioscience, 25–0149-42),
anti-CD19-APC (mouse, BD Biosciences, 555415), anti-CD27-PE (mouse BD
Biosciences, 555441), anti-CD38-APC (mouse, BD Biosciences, 555462),
anti-CD38-FITC (mouse, BD Biosciences, 555459). Surface protein expression was
detected by a BD FACSCalibur cell analyzer (BD Biosciences) and data were
analyzed using the FlowJo software.
Lee S.H., Singh I., Tisdale S., Abdel-Wahab O., Leslie C.S, & Mayr C. (2018). Widespread intronic polyadenylation inactivates tumor suppressor genes in leukemia. Nature, 561(7721), 127-131.
+ Open protocol
+ Expand

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