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4 protocols using trifluoroacetic acid

1

Peptide Synthesis Protocol Optimized

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The following reagents, 9-fluorenylmethoxycarbonyl (Fmoc) protected aa, O-(Benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium tetrafluoroborate (TBTU), N,N′-dicyclohexylcarbodiimide (DDC) and 1-hydroxybenzotrazole (HOBt), were purchased from AAPPTEC (Louisville, KY, USA), ChemMatrix resin (0.60 mmol/g) from PCAS BiomMatrix Inc. (Saint-Jean-sur-Richelieu, QC, Canada), N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIS), Triton X-100, α-cyano-4-hydroxycinnamic acid (HCCA), methanol and absolute ethanol from Sigma-Aldrich (St. Louis, MO, USA), piperidine, pyridine, phenol, potassium cyanide, isopropyl alcohol (IPA), acetonitrile (ACN), N-methyl-2-pyrrolidone (NMP), trifluoroacetic acid (TFA) and diethyl ether from Panreac (Barcelona, Spain), acetic anhydride (Ac2O), ninhydrin and ethanedithiol (EDT) from Merck (Boston, MA, USA) and dichloromethane (DCM) from The Dow Chemical Company (Bogotá, Colombia).
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2

Gelatin-based Hydrogel for MMP-2 Inhibition

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Gelatin from bovine skin type B (gel strength 225 g Bloom), protease from Streptomyces griseus, NaOH, MMP-2 human recombinant, glyoxal solution 40% and glycine were purchased from Sigma-Aldrich (St. Louis, MO, USA). TM and HPMC were obtained from Fagron (Barcelona, Spain). Absolute ethanol, trifluoroacetic acid and acetonitrile were acquired from Panreac (Madrid, Spain). Dialysis membrane with a molecular weight cut-off 3500 g/mol was provided by Medicel Membranes (London, UK). Timabak 0.5% (Thea Laboratories; Madrid, Spain) was purchased in a local pharmacy store. Ultrapure milli-Q water was used in all the studies. All other chemicals were reagent grade and used as received.
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3

Itraconazole Oral Solution Formulation

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The reference product (Itrafungol® oral solution) was acquired from a local pharmacy. Itraconazole and Syrspend pH 4 Dry were purchased from Fagron Ibérica. Polyethylene glycol 400, hydrochloric acid 37%, hydroxypropyl methylcellulose (HPMC), and sodium benzoate were obtained from Sigma Aldrich (Barcelona, Spain). Polysorbate 80, sorbitol liquid 70%, propylene glycol, and sodium carmellose 1500–4500 were obtained from Guinama S.L.U. (Valencia, Spain). Sodium sulfonyl ether β-cyclodextrin was obtained from Fismer Scientific, and acetonitrile (ACN), methanol, and trifluoroacetic acid (TFA) were obtained from Panreac® (Barcelona, Spain).
The amber bottles of 60 mL for the packaging were provided by Guinama S.L.U.
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4

Analytical Standards Procurement for Compound Characterization

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All standards (linalool, bisabolol oxide A, eucalyptol, glycyrrhizic acid and absolute ethanol and methanol solvents) were purchased from Sigma-Aldrich (Steinheim, Germany) (>95% purity). Acetonitrile [for high-performance liquid chromatography (HPLC), 99.9%], trifluoroacetic acid (99.8%) and purified and calcined siliceous earth (USP-NF) RRS-CODEX were purchased from Panreac (Barcelona, Spain). Alkane standard solution C8-C20 was purchased from Fluka (Steinheim, Germany).
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