Peppermintstick phosphoprotein molecular weight standard

Manufactured by Thermo Fisher Scientific

Sourced in United States, Sweden

The PeppermintStick™ Phosphoprotein Molecular Weight Standards is a set of pre-stained protein markers specifically designed for the analysis of phosphorylated proteins using Western blotting techniques. The standards cover a molecular weight range of 10 to 250 kDa and include prestained phosphorylated proteins for easy identification of target proteins.

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Lab products found in correlation

Agarose, by Thermo Fisher Scientific (2 mentions) Acetonitrile, by Merck Group (2 mentions) Iodoacetamide, by Merck Group (2 mentions) Glycine, by Merck Group (2 mentions) Sypro ruby, by Thermo Fisher Scientific (2 mentions) Ficoll paque, by GE Healthcare (2 mentions) Acrylamide, by GE Healthcare (2 mentions) Chaps, by GE Healthcare (2 mentions) Dl dithiothreitol, by Merck Group (2 mentions) Lanthanum chloride, by Merck Group (2 mentions) Sodium dodecyl sulfate (sds), by GE Healthcare (2 mentions) Coomassie blue g 250, by Merck Group (2 mentions) Urea, by GE Healthcare (2 mentions) Ampholine, by GE Healthcare (2 mentions) Pro q diamond dye, by Thermo Fisher Scientific (2 mentions) Glycerol, by GE Healthcare (2 mentions) Sequencing grade modified trypsin, by Promega (1 mentions) Potassium dihydrogen phosphate, by Merck Group (1 mentions) Acetone, by Merck Group (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)

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PeppermintStick (2 citations)

4 protocols using peppermintstick phosphoprotein molecular weight standard

Two-Dimensional Gel Electrophoresis Reagents

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Acetonitrile (ACN), acetone, trifluoroacetic acid (TFA), trichloroacetic acid (TCA), DL-dithiothreitol (DTT), iodoacetamide (IAA), glycine, EDTA, Tris, endonuclease, phosphoprotease and protease inhibitors, lanthanum chloride, potassium dihydrogen phosphate, Coomassie Blue G-250 were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA); urea, CHAPS, SDS, glycerol, acrylamide, ampholine, and Ficoll-Paque™ were purchased from GE Healthcare (Uppsala, Sweden); Agarose, Pro-Q^® Diamond dye, SYPRO^® Ruby and PeppermintStick™ Phosphoprotein Molecular Weight Standards were from Invitrogen™ (Carlsbad, CA, USA); piperazine di-acrylamide (PDA), TEMED, Bio Rad Protein Assay, IPG strips, were from Bio-Rad Laboratories (Hercules, CA, USA). Trypsin (sequencing grade modified) was from Promega (Madison, WI, USA). All solvents used were Ultra-Resi-Analyzed grade.

Rocchetti M.T., Rascio F., Castellano G., Fiorentino M., Netti G.S., Spadaccino F., Ranieri E., Gallone A., Gesualdo L., Stallone G., Pontrelli P, & Grandaliano G. (2020). Altered Phosphorylation of Cytoskeleton Proteins in Peripheral Blood Mononuclear Cells Characterizes Chronic Antibody-Mediated Rejection in Kidney Transplantation. International Journal of Molecular Sciences, 21(18), 6509.

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Comprehensive Phosphoproteomic Sample Preparation

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Acetonitrile (ACN), acetone, trifluoroacetic acid (TFA), trichloroacetic acid (TCA), DL-dithiothreitol (DTT), iodoacetamide (IAA), glycine, EDTA, Tris, endonuclease, phosphoprotease and protease inhibitors, lanthanum chloride, potassium dihydrogen phosphate, Coomassie Blue G-250, imidazole, alkaline phosphatase were purchased from Sigma (Sigma Aldrich St.Louis, MO, USA); urea, CHAPS, SDS, glycerol, acrylamide, ampholine, and Ficoll-Paque™ were purchased from GE healthcare (Uppsala, Sweden); Agarose, Pro-Q® Diamond Phosphoprotein Enrichment kit, Pro-Q® Diamond dye, SYPRO® Ruby and PeppermintStick™ Phosphoprotein Molecular Weight Standards were from Invitrogen™ (Carlsbad, CA); piperazine di-acrylamide (PDA), TEMED, Bio Rad Protein Assay, IPG strips, were from Bio-Rad Laboratories (Hercules, CA). Trypsin (sequencing grade modified) was from Promega (Madison, Wisconsin, USA). All solvents used were Ultra-Resi-Analyzed grade.

Rocchetti M.T., Alfarano M., Varraso L., Di Paolo S., Papale M., Ranieri E., Grandaliano G, & Gesualdo L. (2014). Two dimensional gel phosphoproteome of peripheral blood mononuclear cells: comparison between two enrichment methods. Proteome Science, 12, 46.

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Quantifying Phosphoproteins in LV Lysate

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20 μg of LV free wall lysate, prepared as outlined above, was resolved on 4–20% SDS gels and stained for total phosphoprotein with Pro-Q Diamond stain according to the manufacturer’s instructions (ThermoFisher). PeppermintStick phosphoprotein molecular weight standard was used to determine protein sizes and as positive/negative controls for correct image adjustment (ThermoFisher). Total protein was determined by staining with Coomassie blue. Gels were imaged using UV transillumination on a G-Box Chemi-XR5 chemiluminescent imaging system (Syngene). Specific gel bands were quantified using ImageJ (NIH).

Pappas C.T., Farman G.P., Mayfield R.M., Konhilas J.P, & Gregorio C.C. (2018). Cardiac-Specific Knockout of Lmod2 Results in a Severe Reduction in Myofilament Force Production and Rapid Cardiac Failure. Journal of molecular and cellular cardiology, 122, 88-97.

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Characterizing Protein Phosphorylation in Cells

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oCOm‐21 and BP‐21 (CO‐depleted oCOm‐21 byproduct), were synthesized by the Larsen group, Department of Chemistry, University of Otago (Kueh et al., 2017 (link)). Levosimendan (L5545) and hemopexin (H9291), phenylmethylsulfonyl fluoride (78830), Protease Inhibitor Cocktail (P8340), Phosphatase Inhibitor Cocktail (P5726), oxalic acid, and Spurr's epoxy resin were purchased from Sigma‐Aldrich. HALT protease and phosphatase inhibitor cocktail 100× (78430), Triton X‐100 (28314), Pro‐Q™ Diamond Phosphoprotein gel stain (P33300), SYPRO® Ruby Protein Gel Stain (S12000), and PeppermintStick™ Phosphoprotein molecular weight Standard (P27167) were purchased from Thermo Fisher Scientific.

Payne F.M., Nie S., Diffee G.M., Wilkins G.T., Larsen D.S., Harrison J.C., Baldi J.C, & Sammut I.A. (2024). The carbon monoxide prodrug oCOm‐21 increases Ca2+ sensitivity of the cardiac myofilament. Physiological Reports, 12(6), e15974.

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