Sitaz

Manufactured by Thermo Fisher Scientific

The SiTAZ is a laboratory equipment designed for the detection and analysis of silicon-based compounds. It utilizes advanced spectroscopic techniques to provide precise measurements and data on the presence and concentration of silicon-containing materials in various samples. The core function of the SiTAZ is to enable accurate and reliable silicon analysis in a wide range of research and industrial applications.

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Lab products found in correlation

2 protocols using sitaz

siRNA Knockdown of YAP and TAZ

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Cells were plated in a 6-well culture plate. When cells reached 60% confluence, cells were transfected with siRNA (siCon, siYAP, siTAZ, or siYAP/TAZ) and Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific) diluted in Opti-MEM (Gibco, Grand Island, NY). siCon, siYAP (cat AM16708, assay ID 17374), siTAZ (cat AM16708, assay ID 23134) were purchased from ThermoFisher Scientific. Detail dosage and procedures were conducted according to the manufacturer’s protocol. After 48 hours of transfection, cells were harvested for RNA analysis.

Zhang Y., Xu H., Cui G., Liang B., Chen X., Ko S., Affo S., Song X., Liao Y., Feng J., Wang P., Wang H., Xu M., Wang J., Pes G.M., Ribback S., Zeng Y., Singhi A., Schwabe R.F., Monga S.P., Evert M., Tang L., Calvisi D.F, & Chen X. (2022). β-Catenin sustains and is required for YES-associated protein oncogenic activity in cholangiocarcinoma. Gastroenterology, 163(2), 481-494.

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Molecular Mechanisms of HCC Cell Lines

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The SNU449 and SUN475 human HCC cell lines were a kind gift from Dr John Gordan at the University of California, San Francisco (San Francisco, CA). PLC/PRF/5 and Focus cells were purchased from American Type Culture Collection (Manassas, VA). All cell lines were authenticated (Genetica DNA Laboratories, Burlington, NC) and tested clear of mycoplasma contamination. Cells were cultured in Dulbecco’s modified Eagle medium (Gibco, Grand Island, NY) with 5% fetal bovine serum (Gibco), 100 μg/mL streptomycin, and 100 U/mL penicillin at 37°C in a 5% CO₂ humidified incubator. For siRNA transfection, SNU449, SNU475, PLC/PRF/5, and Focus were transfected with siSC, siYAP (cat AM16708, assay ID 17374; Thermofisher Scientific), siTAZ (cat AM16708, assay ID 23134; Thermofisher Scientific), or siYAP/siTAZ by using the Thermofisher siRNA Transfection Kit. Procedures were conducted as indicated by the manufacturer. After 72 hours of transfection, cells were harvested for Western blot or RNA analysis. Proliferation assays were conducted as before.³⁵ (link)

Wang H., Wang J., Zhang S., Jia J., Liu X., Zhang J., Wang P., Song X., Che L., Liu K., Ribback S., Cigliano A., Evert M., Wu H., Calvisi D.F., Zeng Y, & Chen X. (2020). Distinct and Overlapping Roles of Hippo Effectors YAP and TAZ During Human and Mouse Hepatocarcinogenesis. Cellular and Molecular Gastroenterology and Hepatology, 11(4), 1095-1117.

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