Anti cd31 antibody sc 376764

Resomer RG 503 was used as a commercial source of PLGA (Boehringer, Rhein Ingelheim, Germany) with a 50:50 lactide to glycolide ratio and a molecular weight of 33,000 Da. Dimethyl sulfoxide (DMSO), PTX, and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) solvents were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
Fetal bovine serum, antibiotics (penicillin-streptomycin), trypsin-ethylenediaminetetraacetic acid, trypan blue stain, and all medium additives were obtained from Life Technologies (Gaithersburg, MD, USA). An enhanced chemiluminescence kit was purchased from Amersham (Arlington Heights, IL, USA). An anti-CD31 antibody (sc-376764) was acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies specific for cleaved-PARP (#5625), PCNA (#2586) and Ki-67 (#9027) were obtained from Cell Signaling Technology (Danvers, MA, USA).

This assay was performed by methods described previously with modification [60 (link)]. Mice were injected subcutaneously with 0.5 mL Matrigel (BD Biosciences, San Jose, Santa Clara, CA, USA) containing 100 ng recombinant murine VEGF (R&D Systems, Minneapolis, MN, USA). Controls did not contain VEGF. After 10 days, the mice were sacrificed and plugs were harvested from underneath the skin. The plugs were fixed, embedded, and sectioned. To visualize capillaries, samples were immunohistochemically stained with anti-CD31 antibody (sc-376764, Santa Cruz, Santa Cruz, CA, USA).