The antibodies used in this study are as follows: WRN (8H3) mouse mAb (4666, 1/1,000 dilution; Cell Signaling), mouse anti-FLAG (F1804; Sigma-Aldrich, 1/1,000 [immunoblotting] or 1/500 [immunofluorescence] dilution), rabbit anti–phospho-histone H2A.X (Ser139) (2577, 1/800 dilution; Cell Signaling), mouse anti-GAPDH (ab8245, 1/30,000 dilution; Abcam), mouse Alexa Fluor 488 (1/1,000 dilution; Molecular Probes) and secondary rabbit (P0448, 1/1,000 dilution; Dako), mouse anti-IgG-HRP (P0161, 1/1,000 dilution; Dako).
Mouse anti gapdh ab8245
Manufactured by Abcam
Sourced in United States
Mouse anti-GAPDH (ab8245) is a primary antibody that recognizes the GAPDH protein. GAPDH is a glycolytic enzyme involved in the metabolic process. This antibody can be used in various immunological techniques to detect and study the GAPDH protein.
Automatically generated - may contain errors
Lab products found in correlation
P0448, by Agilent Technologies (1 mentions)
Mouse anti flag f1804, by Merck Group (1 mentions)
Mouse alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Wrn 8h3 mouse mab, by Cell Signaling Technology (1 mentions)
Anti mouse igg hrp, by Agilent Technologies (1 mentions)
Rabbit anti phospho histone h2a x ser139, by Cell Signaling Technology (1 mentions)
Anti rabbit na934v, by GE Healthcare (1 mentions)
β nad, by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Rabbit anti aurora a, by Cell Signaling Technology (1 mentions)
Hoechst nuclear dye, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated secondary antisera, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti hsp70 sc 33 575, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti pcna 13110, by Cell Signaling Technology (1 mentions)
Chemidoc mp imaging system, by Bio-Rad (1 mentions)
5 protocols using mouse anti gapdh ab8245
1
Immunostaining Antibody Dilutions
2
Antibody Sourcing for Protein Analysis
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Rabbit anti-ERp29 (ab11420), mouse monoclonal anti-Proinsulin (ab8304), rabbit anti-Sec24D (ab191566), and mouse anti-GAPDH (ab8245) were purchased from Abcam (Cambridge, MA). Mouse monoclonal anti-Proinsulin was purchased from Cell Signaling Technologies (Danvers, MA, Catalog L6B10) and from Sigma-Aldrich (St. Louis, MO, Catalog SAB4200691). Rat monoclonal anti-C-peptide (NBP-05439) was purchased from Novus (Centennial, CO). Mouse anti-BiP (610979) was purchased from BD Biosciences (Franklin Lakes, NJ). Horseradish peroxidase-conjugated secondary antibodies (anti-mouse, NA931V and anti-rabbit, NA934V) were from GE Healthcare (Pittsburgh, PA).
3
Characterization of SNAP25 Antibodies
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The following primary antibodies were used in this study (Table 1 ): mouse anti-SNAP25206/197 (SMI-81R) (Covance, Princeton, NJ); mouse anti-SNAP25206/197 (MC-6050) and mouse anti-SNAP25197 (MC-6053) (R&D Antibodies, Las Vegas, NV, USA); human recombinant anti-SNAP25197 (Ab632), mouse recombinant anti-SNAP25197 (Ab635) and rabbit anti-SNAP25197 (RGT-1092) (Allergan, Irvine, CA, USA); mouse anti-GAPDH (ab8245) (Abcam, Cambridge, MA, USA); rabbit anti-protein gene product 9.5 (PGP9.5; AbD Serotec, Raleigh, NC, USA).
4
Characterizing Aurora-A Kinase Regulation
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For Western blot analysis, the following antibodies were used: rat anti-PARP10 (sc-53858) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse anti-Aurora-A (15815719) from Thermo Fisher Scientific (Waltham, MA, USA); rabbit anti-phospho-T288 Aurora-A (#3079) and rabbit anti-MAR/PAR (#83372) from Cell Signalling Technology (Beverly, MA, USA); and mouse anti-GAPDH (ab8245) from AbCam (Cambridge, UK). Secondary antibodies were obtained from Merck (Darmstadt, Germany). For immunofluorescence analysis, the following antibodies were used: rabbit anti-Aurora-A (#14475) from Cell Signalling Technology (Beverly, MA, USA); mouse anti-Pericentrin (ab28144) from AbCam (Cambridge, UK); and rabbit anti-phospho-Ser10 Histone-H3 (06/570) form Merck (Darmstadt, Germany). Hoechst nuclear dye and RNAse were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Propidium Iodide (PI), Thymidine, PJ34, β-NAD+ and ATP were sourced from Merck (Darmstadt, Germany). OUL35 [47 (link)] was kindly provided by Prof. Lari Lehtio (University of Oulu, Finland) and Prof. Oriana Tabarrini (University of Perugia, Italy).
5
Western Blot Analysis of Cellular Proteins
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Cells were lysed in Tris-HCl pH 7.6, 50 mM, deoxycholic acid sodium salt 0.5%, NaCl 140 mM, NP40 1%, EDTA 5 mM, NaF 100 mM, Na pyrophosphate 2 mM and protease inhibitors. For phospho p65, 2 mM Na orthovanadate and 5 mM Na butyrate were also added. Lysates were separated on 8% or 10% acrylamide gel and immunoblotted using standard procedures. Rabbit anti-Hsp70, sc-33575 (Santa Cruz Biotechnology, CA), rabbit anti-PCNA, #13110 (Cell Signaling Technology Inc., Danvers, MA); mouse anti-b3-tubulin (TU-20), #4466 (Cell Signaling Technology Inc, Danvers, MA); mouse anti-GFAP, MAB360 (Merck Millipore, Darmstadt), rabbit anti-p65, #3034S (Cell Signaling Technology Inc, Danvers, MA); rabbit anti-phospho p65 (P-p65), #3033S (Cell Signaling Technology Inc, Danvers, MA); mouse anti-GAPDH, ab8245 (AbCam, Cambridge, UK) and HRP-conjugated secondary antisera (Santa Cruz Biotechnology, CA) were used followed by enhanced chemiluminescence (ECL Amersham, Amersham, UK) and images were acquired using the BioRad ChemiDoc MP Imaging System (BioRad, Hercules, CA). Densitometric analysis was performed using the BioRad associated Image Lab Software (BioRad, Hercules, CA). Values are expressed as fold over internal control, represented by GAPDH, that does not change significantly in the proteome profiles.
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