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3 protocols using 4 4 diaminodiphenylsulfone

1

Epoxy Resin Synthesis and Characterization

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C2-C8 diepoxy monomer and 4,4′-diaminodiphenylsulfone (purchased by Sigma-Aldrich) were mixed by the mol ratio of 1:1 and then heated to 180 °C for 3 mins until it formed a liquid-phase mixture. Next, we transferred a droplet of the liquid-phase mixture onto the Al/a-SiO2 substrate and covered the sample with a coverslip. The procedure for substrate preparation and measurement is the same in our previous work (29 ), where we coated ∼80 nm of Al on a polished fused silica wafer and the thickness of Al is measured by time-domain thermoreflectance (TDTR) instrument (picosecond acoustics). The thickness of epoxy resin was ∼0.2 mm. The epoxy resins were post cured at 180 °C for 24 h (29 ).
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2

Reagents for Cell Death Assays

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Deferoxamine, ferrostatin-1, erastin, sulfacetamide, sulfasalazine (SSZ), zymosan, diphenyleneiodonium chloride (DPI), 4,4′-diaminodiphenyl sulfone (DDS), sulfisoxazole, tBHP, and sulfasalazine were purchased from Sigma. Phorbol 12-myristate 13-acetate (PMA), sulfamethoxazole, sulfanilamide, sulfapyridine, 5-aminosalicylic acid (5-ASA), sulfadimidine, sulfametoxydiazine, sulfisomidine, sulfanitran, sulfanilamide, and piroxicam were purchased from Wako. We purchased 1-O-hexadecyl-2-arachidonoyl-sn-glycero-3-phosphocholine (ether-linked phosphatidylcholine) from Avanti polar lipids. Necrostatin-1 was purchased from FOCUS Biomolecules, and 2-mercaptoethanol was purchased from MP Biomedicals. z-VAD-fmk was purchased from Peptide institute. Trolox, sulfadiazine, sulfadimethoxine, sulfadoxin, and sulfamethoxypyridazine were purchased from Tokyo Chemical Industry. Cl-amidine was purchased from Cayman chemicals. Ionomycin was purchased from Merck Millipore.
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3

Synthesis of Epoxy Resin Composites

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1H-imidazole, 3-chloroperoxybenzoic acid (77%), 3-(aminomethyl)-3,5,5-trimethylcyclohexanamine (IPD), 4,4’-diaminodiphenylsulfone (DDS), and trimethyl-1,6-hexanediamine (TMHMDA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 4-bromo-1-butene and lithium bis(trifluoromethane)sulfonimide were purchased from ABCR (Karlsruhe, Germany). 4-methyl-hexahydro-phthalic anhydride (MHHPA) and bisphenol A diglycidyl ether (DGEBA) were supplied by Huntsman (The Woodlands, TX, USA). N,N-dimethylbenzylamine (BDMA) was purchased from Fisher Scientific (Hampton, NH, USA). All chemicals were used without further purification.
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