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Dihydroethidium dhe s0063

Manufactured by Beyotime
Sourced in China

Dihydroethidium (DHE) (S0063) is a fluorogenic dye used for the detection of superoxide radicals in biological samples. It functions as an oxidative stress indicator by undergoing a reaction with superoxide, resulting in the formation of a fluorescent product. The core function of this product is to provide a method for the quantitative measurement of superoxide levels in various cellular systems.

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2 protocols using dihydroethidium dhe s0063

1

Molecular Mechanisms of Neuroinflammation

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MI-2 (S7429) was purchased from Selleckchem (Houston, Texas, USA); Human recombinant MALT1 protein (hrMALT1) (ab271604) and Luxol Fast Blue (LFB) Staining Kit (ab150675) were purchased from Abcam (Cambridge, UK). Nissl Staining Solution (C0117) and Dihydroethidium (DHE) (S0063) were purchased from Beyotime (Shanghai, China). Silver Staining Kit (G1052) was purchase from Servicebio (Wuhan, China). Evans blue (DK0001) was purchase from Leagene (Beijing, China). Antibodies: p-p65 (phos-NF-κB, ab194726), Bip (ab21685), CD31 (ab64543), GFAP (ab279290), NeuN (ab104224), AQP4 (ab259318), MMP9 (ab76003), IL-1β (ab254360), ZO-1 (ab221547), Donkey Anti-Goat IgG H&L (Cy5) (ab6566), Donkey Anti-Rabbit IgG H&L (Alexa Fluor 647) (ab150075), Donkey Anti-Goat IgG H&L (Alexa Fluor 488) (ab150129), Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) and Donkey Anti-Rabbit IgG H&L (Alexa Fluor 488) (ab150073) were purchased from Abcam (Cambridge, UK). MALT1 (GB11790) and Iba-1 (GB12105) were purchased from Servicebio (Wuhan, China). CD86 (NBP2-67417) was purchased from Novus (Minneapolis, USA).
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2

Examining Molecular Pathways in Cellular Dysfunction

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RU.521 (S6841) and SR-717 (S0853) were purchased from Selleckchem (Houston, Texas, USA); 4-PBA (S305161) was purchased from Aladdin (Shanghai, China). Hematoxylin and Eosin (HE) Staining Kit (C0105S), Dihydroethidium (DHE) (S0063), and Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick End-Labeling (TUNEL) Apoptosis Assay Kit (C1089) were purchased from Beyotime (Shanghai, China). Antibodies: cGAS (ab252416), STING (ab13647), BiP (ab21685), Goat Anti-Rabbit IgG (ab150077), and Goat Anti-Mouse IgG (ab150115) were purchased from Abcam (Cambridge, UK); SP-C (SC-13979) was purchased from Santa Cruz Biotechnology (Dallas, USA); β-actin (3700) were purchased from Cell Signaling Technology (Boston, USA).
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