The sodium phosphate buffer was prepared using di‐sodium hydrogen phosphate anhydrous (Na2HPO4, purity 99.0%) and sodium phosphate monobasic (NaH2PO4, purity 99–100.5%) both acquired at Panreac. Ammonium sulfate (purity 99%) and sodium azide (purity 99%) were purchased from Panreac and Acros Organics, respectively.
Sodium azide
Manufactured by ITW Reagents
Sourced in Spain
Sodium azide is a chemical compound with the formula NaN3. It is a white, crystalline solid that is commonly used as a preservative and as a reagent in various laboratory applications. Sodium azide has a range of functions, including its use as a preservative and in the synthesis of other compounds. However, a detailed description of its core functions would require additional context that is not provided in the request.
Automatically generated - may contain errors
Lab products found in correlation
Milli q system, by Merck Group (2 mentions)
2 mercaptoethanol, by Merck Group (2 mentions)
Sodium bicarbonate, by ITW Reagents (2 mentions)
2 4 dinitrophenylhydrazine, by ITW Reagents (2 mentions)
Hydrochloric acid (hcl), by ITW Reagents (2 mentions)
Ammonium sulfate, by ITW Reagents (1 mentions)
Antimycin a, by Merck Group (1 mentions)
Oxygen electrode, by Hansatech (1 mentions)
Analytical grade methanol, by Merck Group (1 mentions)
Potassium phosphate, by ITW Reagents (1 mentions)
Calcium chloride, by ITW Reagents (1 mentions)
Fluoro carbonyl cyanide phenylhydrazone (fccp), by Merck Group (1 mentions)
Potassium cyanide, by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Rotenone, by Merck Group (1 mentions)
Antimycin, by Merck Group (1 mentions)
Atovaquone, by GlaxoSmithKline (1 mentions)
Trichloroacetic acid tca, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
8 protocols using sodium azide
1
Sodium Phosphate Buffer Preparation
2
Measuring Respiratory Activity in C. albicans
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C. albicans strains were grown in YPG at 30°C until they reached an O.D. ≈1–1.5. Cells from 20–30 mL cultures were recovered by low speed centrifugation (5,000 rpm, 3 min), washed twice with PBS and suspended in 1 mL PBS. To quantify oxygen consumption, an oxygen electrode (Hansatech Instruments) was used. The oxygen electrode chamber was maintained at 30°C, filled with 1 mL PBS and 10 μL of the cell suspension was introduced in the electron chamber. Different compounds that inhibit specific complexes of the electron transport chain were added to the electrode chamber to quantify the contribution of different complexes to the respiration rate. Antimycin A (Sigma-Aldrich) and sodium azide (Panreac) were added at the concentration indicated in the figures to the electron chamber. The relative respiration rate was determined by dividing the respiration rate in the presence of the inhibitor by the respiration rate in its absence (basal).
3
Quantification of Antibiotics in Samples
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Primary standards of Ampicillin trihydrate (99.0%, AMP), Benzathine penicillin G tretrahydrate (97.2%, PNG), Dicloxacillin sodium hydrate (99.0%, DCL), Oxacillin salt hydrate (99.0%, OXA), Tetracycline hydrochloride (97.7%, TC), Doxycycline hyclate (98.7%, DXC), Chlortetracycline hydrochloride (94.6%, CTC) and Oxytetracycline hydrochloride (95.0%, OTC) were supplied by Dr. Ehrenstofer GmbH (Germany). The 2,4-Dinitrophenylhydrazine, potassium phosphate, sodium azide, hydrochloric acid, sodium bicarbonate and calcium chloride were purchased from PanReac (Barcelona, Spain). Analytical grade methanol, sodium hydroxide EMSURE (99%), biotechnological grade sodium chloride, 2-mercaptoethanol, and coomassie blue brilliant G250 (CBB) were purchased from Merck (Darmstadt, Germany). Bovine serum albumin (BSA) free of fatty acids (98%) was supplied from Sigma Aldrich (San Luis, USA). Water was purified with a Milli-Q system (Millipore, Bedford, MA, USA).
4
Mitochondrial Respiration Inhibitor Screening
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Atovaquone, endochin and TCAMS compounds (GSK), stigmatellin (Fluka), FCCP (Sigma) and proguanil (Molekula) were dissolved in DMSO. Sodium azide (Panreac), potassium cyanide (Sigma) and chloroquine (Sigma) in water and myxothiazol, antimycin and rotenone (Sigma) in ethanol. In the assay, the final concentration for DMSO or ethanol was 0.5% (v/v), a concentration that did not inhibit oxygen consumption and which was used as a 0% inhibition control (Control 1).
5
Fluoroquinolone Antibiotics Quantification Protocol
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The following reagents were of analytical standard grade and Sigma-Aldrich (Saint Louis, MO, USA) supplied: danofloxacin, enrofloxacin, ciprofloxacin, norfloxacin, sarafloxacin, bovine serum albumin (BSA), pepsin, α-chymotrypsin and trypsin. SDS (Pepyn, France) supplied methanol (MeOH) and acetonitrile (MeCN) (HPLC grade). PanReac® (Barcelona, Spain) supplied 2,4-Dinitrophenylhydrazine, potassium phosphate, sodium azide, hydrochloric acid, sodium bicarbonate and calcium chloride. Sodium hydroxide, sodium chloride, potassium chloride, 2-mercaptoethanol, Coomassie blue-brillant G250 and trichloroacetic acid (TCA) (99.5%) were purchased from Merck® (Darmstadt, Germany). Proteomic or molecular biology grade reagents: sodium dodecyl sulfate, Tris Base and Glycerol were supplied from Amresco® (West Chester, PA, USA). Orthophosphoric acid, molecular weight marker Spectra Multicolor Broad Range, Rabbit anti-DNP monoclonal and goat anti-Rabbit HRP antibodies were purchased from Scharlab (Barcelona, Spain), Thermo Scientific (Waltham, MA, USA), Invitrogen (Carlsbad, MA, USA) and Bioss Inc. (Woburn, MA, USA), respectively. Water was purified with a Milli-Q system (Millipore, Bedford, MA, USA).
6
Emulsions with Soybean-Walnut Oil and Inulin
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The emulsions were prepared using, as a dispersed phase, soybean‐and‐walnut oil (La Española, Seville, Spain; 250 g kg−1) purchased from a local market. This oil contains 60 g kg−1 ALA. Tween 80 (hydrophilic–lipophilic balance value 15; 25 g kg−1), supplied by Sigma Aldrich, Spain was used as an emulsifier. Inulin fibre Frutafit® (60 g kg−1), kindly provided by Brenntag, was also used. Sodium azide (Panreac, Montcada, Spain) (0.2 g kg−1) was added to the samples as a preservative. To complete the emulsion formulation, Milli‐Q water was used.
In order to prepare the nanoemulgels, Milli‐Q water and high‐performance xanthan gum, KELTROL® Advanced Performance, provided by CP Kelco Company (San Diego, CA) were used.
In order to prepare the nanoemulgels, Milli‐Q water and high‐performance xanthan gum, KELTROL® Advanced Performance, provided by CP Kelco Company (San Diego, CA) were used.
7
Polymer Membrane Fabrication and Functionalization
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Poly-l -lactide (PLLA) (Corbion, Amsterdam, The Netherlands) was used to prepare membranes. Chloroform (>98% Macron Fine Chemicals, Gliwice, Poland), tert-butanol (tBuOH), ethanol (EtOH), methanol (MeOH, 98%), acetonitrinile (99%, Panreac, Darmstadt, Germany), dichloromethane (CH2Cl2, 98%, Macron Fine Chemicals), toluene (99%, Sigma Aldrich, St. Louis, MO, USA), N,N-dimethylformamide (DMF, 99%, Macron Fine Chemicals), acetic acid (Sigma Aldrich), deuterated chloroform (99.8% Sigma Aldrich) and Milli Q water were used as a solvents. Sodium hydroxide (99%, Panreac), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC·HCl, 98%), N-hydroxysuccinimide (NHS, 98%), triethylamine (<99.5%), propargylamine (97.5%), 3-bromopropylamine hydrobromide (98%), sodium azide (NaN3, 99.5%), copper (II) sulphate pentahydrate (Cu2SO4·5H2O, 98%), sodium ascorbate (98%), sodium sulphate anhydrous (Na2SO4, 96%, Panreac), N,N-diisopropylethylamine (DIPEA, 99%), N,N′-dicyclohexylcarbodiimide (DCC, 99%), 1-hydroxybenzotriazole hydrate (HOBT, 97%), O-toluidine blue (TBO, dye content 80%), dansyl chloride (>99%), indomethacin (>99%), thionyl chloride (>99%) from Sigma Aldrich were used as reactants. All the synthesized products spectra are summarized in Supplementary Information (Schemes S1–S4, Figures S1–S8) .
8
Pectin-Stabilized Orange Oil Emulsions
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Orange essential oil (5 wt%), kindly provided by Bidah y Chaumel S.L. (Altorreal, Spain) was utilized as the dispersed phase. The nonionic surfactant T80 (HLB 15), from 1 to 5 wt%, supplied by Sigma Aldrich (Madrid, Spain), was used as an emulsifier. Sodium azide (0.1 wt%) from Panreac (Barcelona, Spain) was added to the samples to preserve them. In order to complete the emulsions formulation, Milli-Q water was used. The formulations studied were coded EX, where X is the T80 concentration expressed as percentage by weight.
In order to obtain stable and non-Newtonian emulsions, high methoxyl pectin from lemon peel (methoxylation degree 65.8%), provided by Silvateam (Valli, Italy), was used as a thickening agent.
In order to obtain stable and non-Newtonian emulsions, high methoxyl pectin from lemon peel (methoxylation degree 65.8%), provided by Silvateam (Valli, Italy), was used as a thickening agent.
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