Three lines of hiPSCs were purchased from the American Type Culture Collection (ATCC). Two lines, HYS0103 (ATCC® ACS1020™) and KYOUDXR0109B [201B7] (ATCC® ACS1023™), were classified as ‘normal’ (no known disease background), and DYP0730 (ATCC® ACS1003™) were generated from a donor with Down Syndrome.
Acs 1003
Manufactured by American Type Culture Collection
Sourced in United States
The ACS-1003 is a compact, benchtop autoclave designed for sterilizing laboratory equipment and materials. It features a stainless steel chamber with a capacity of up to 3 liters. The autoclave operates at temperatures up to 121°C and pressures up to 15 psi, ensuring effective sterilization. The ACS-1003 includes safety features such as automatic shut-off and pressure release mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
Acs 1023, by American Type Culture Collection (1 mentions)
Hipscs, by American Type Culture Collection (1 mentions)
Dmem f12, by Corning (1 mentions)
Rock inhibitor y 27632, by Abcam (1 mentions)
Matrigel solution, by Corning (1 mentions)
Kyou dxr0109b, by American Type Culture Collection (1 mentions)
Mtesr1 medium, by STEMCELL (1 mentions)
2 protocols using acs 1003
1
Generation of hiPSC Lines from Donors
2
Reprogramming Adult Cells to Induced Pluripotent Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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iPS-KYOU, has been obtained in the Shinya Yamanaka laboratory (Kyoto University, Japan) by retroviral reprogramming of adult female skin fibroblasts. The iPS-KYOU cell line was purchased from the ATCC cell bank (KYOU-DXR0109B, ACS-1023™, ATCC®, United States).
iPS-AFS17, human Induced Pluripotent Stem Cells, obtained by lentiviral reprogramming of amniotic fluid stem cells by Dashinimaev et al. (2017) (link).
iPS-DP human Induced Pluripotent Stem Cells, obtained by lentiviral reprogramming of dermal papilla cells (Muchkaeva et al., 2014 (link)).
iPS-DYP0730, human Induced Pluripotent Stem Cells, derived from dermal fibroblasts obtained from ATCC CCL-54 Detroit 532, a human Down syndrome cell line. The iPS-DYP0730 cell line was purchased from the ATCC cell bank (ACS-1003™, ATCC®, United States).
For all pluripotent stem cell passaging, we used ACCUTASE™ сell detachment solution (Stem Cell Technologies) and Rock-inhibitor Y-27632 (5 μM; Abcam), the plastic surface being pre-coated with Matrigel solution (1/40 in DMEM/F12) (Corning). The cells were cultured in mTeSR™1 medium (Stem Cell Technologies) at 37°C in a CO2-incubator with 5% CO2 and 100% humidity.
iPS-AFS17, human Induced Pluripotent Stem Cells, obtained by lentiviral reprogramming of amniotic fluid stem cells by Dashinimaev et al. (2017) (link).
iPS-DP human Induced Pluripotent Stem Cells, obtained by lentiviral reprogramming of dermal papilla cells (Muchkaeva et al., 2014 (link)).
iPS-DYP0730, human Induced Pluripotent Stem Cells, derived from dermal fibroblasts obtained from ATCC CCL-54 Detroit 532, a human Down syndrome cell line. The iPS-DYP0730 cell line was purchased from the ATCC cell bank (ACS-1003™, ATCC®, United States).
For all pluripotent stem cell passaging, we used ACCUTASE™ сell detachment solution (Stem Cell Technologies) and Rock-inhibitor Y-27632 (5 μM; Abcam), the plastic surface being pre-coated with Matrigel solution (1/40 in DMEM/F12) (Corning). The cells were cultured in mTeSR™1 medium (Stem Cell Technologies) at 37°C in a CO2-incubator with 5% CO2 and 100% humidity.
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