Na k atpase assay kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Na+/K+-ATPase assay kit is a laboratory tool designed to measure the activity of the sodium-potassium adenosine triphosphatase (Na+/K+-ATPase) enzyme. Na+/K+-ATPase is a crucial membrane-bound enzyme responsible for maintaining the electrochemical gradient across the cell membrane by actively transporting sodium and potassium ions. This assay kit provides a quantitative method to determine the enzymatic activity of Na+/K+-ATPase in various biological samples.

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ATPase assay kit (15 citations) ATPase kit (8 citations) Na+-K+-ATPase (7 citations) Na+/K+-ATPase Kit (5 citations) Na/K-ATPase activity assay kit (3 citations) K+ Assay Kit (2 citations)

11 protocols using na k atpase assay kit

Jejunum Alkaline Phosphatase and ATPase Assay

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Jejunum mucosa alkaline phosphatase (AKP) was measured using an alkaline phosphatase assay kit (no. A059-2-2), and sodium/potassium-transporting adenosine triphosphatase (Na⁺/K⁺-ATPase) activity was measured using the Na⁺/K⁺-ATPase assay kit (no. A070-2-2) according to the instructions of the manufacturer (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

Tang X, & Xiong K. (2022). Intrauterine Growth Retardation Affects Intestinal Health of Suckling Piglets via Altering Intestinal Antioxidant Capacity, Glucose Uptake, Tight Junction, and Immune Responses. Oxidative Medicine and Cellular Longevity, 2022, 2644205.

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Analysis of ATPase Activities

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The activities of H⁺-ATPase, Ca²⁺-ATPase, and Na⁺-K⁺-ATPase were analyzed by using Ca²⁺-ATPase and Na⁺-K⁺–ATPase assay kit (Nanjing Jiancheng, Nanjing, China), following the manufacturer’s protocols.

Qian C., Ji Z., Lin C., Zhang M., Zhang J., Kan J., Liu J., Jin C., Xiao L, & Qi X. (2022). Nitric Oxide Extends the Postharvest Life of Water Bamboo Shoots Partly by Maintaining Mitochondrial Structure and Energy Metabolism. International Journal of Molecular Sciences, 23(3), 1607.

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Exosome Isolation and Characterization Protocol

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Flow meter (Changzhou Chengfeng Flow Meter Company, Changzhou, China); pressure gauge (Yangquan Precision Instrument Factory, China); Cy-3 digital oxygen analyzer (Shanghai Huaguang Instrument Factory, Shanghai, China); BC-2800 blood count instrument (Mindray Medical International, Shenzen, China); 37°C constant temperature water bath (GSY-II; Beijing Medical Equipment Factory, Beijing, China); low speed M109077 automatic centrifuge (Zhongxi, Shanghai, China); 200 L and 1000 L samplers (Eppendorf, Hamburg, Germany); AB fresh/frozen plasma and defoaming agent were donated from blood bank; 2,3-DPG detection kit (Wuhan Huamei Biotechnology Company, Wuhan, China); Na + -K + -ATPase assay kit (Nanjing Jiancheng Biological Company, Nanjing, China); rabbit anti-human CD63 antibody, rabbit anti-human CD81 antibody (System Biosciences, Palo Alto, USA); H7600 transmission electron microscope (TEM) (Hitachi, Tokyo, Japan); and exosome extraction kit (Sigma-Aldrich, St. Louis, USA).

Duan L.S., Liu Y., Li Z.Z., Wang H., Zhou X.F., Wang X.X., Zhang Z.W., Kang Y.Q., Su Y.J, & Guo J.R. (2021). The effect of different storage times on the oxygen-carrying capacity of the exosomes of red blood cells. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 30(4).

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Membrane ATPase Activity Assays

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The serum lactate levels, FFA concentrations, sodium-potassium-ATPase (Na+-K+-ATPase) activities, calcium-magnesium-ATPase (Ca2+-Mg2+-ATPase) activities, and the erythrocyte membrane ATPase activities were detected by colorimetry. Lactic acid assay kit, Non-esterified free fatty acids assay kit, Na+-K+-ATPase assay kit, and Ca2+-Mg2+-ATPase assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The experimental steps were in strict accordance with the manufacturer's instructions.

Yang C., Zhao D., Liu G., Zheng H., Yang H., Yang S, & Yang P. (2018). Atorvastatin Attenuates Metabolic Remodeling in Ischemic Myocardium through the Downregulation of UCP2 Expression. International Journal of Medical Sciences, 15(5), 517-527.

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Cardiac Metabolic Enzyme Activities

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The lactate content and the activities of Na⁺/K⁺-ATPase and Ca²⁺-ATPase in the border-area myocardium were determined with a lactate assay kit, an Na⁺/K⁺-ATPase assay kit, and a Ca²⁺-ATPase assay kit, respectively (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). The procedures were conducted in accordance with the kit instructions strictly.

Cheng W., Wang L., Yang T., Wu A., Wang B., Li T., Lu Z., Yang J., Li Y., Jiang Y., Wu X., Meng H, & Zhao M. (2020). Qiliqiangxin Capsules Optimize Cardiac Metabolism Flexibility in Rats With Heart Failure After Myocardial Infarction. Frontiers in Physiology, 11, 805.

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Quantification of Muscle Metabolic Markers

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Na⁺-K⁺-ATPase activity, cytochrome c oxidase and ATP level were determined according to the manufacturer’s protocol. Briefly, the fresh TA muscles dissected from the left lower limb were homogenized using High-throughput Tissue Grinder (SCIENTZ-48, Scientz Biotechnology, Ningbo, China). The Na⁺-K⁺-ATPase activity, CcO activity and ATP level of homogenates were measured using Na⁺-K⁺-ATPase assay kit, cytochrome C oxidase assay kit or ATP assay kit (Nanjing Jiancheng Bioengineering Institute) by microplate reader (BioTek Synergy H1, United States).

Zhang H., Zhao C., Hou J., Su P., Yang Y., Xia B., Zhao X., He R., Wang L., Cao C., Liu T, & Tian J. (2022). Red ginseng extract improves skeletal muscle energy metabolism and mitochondrial function in chronic fatigue mice. Frontiers in Pharmacology, 13, 1077249.

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Hippocampal Mitochondrial Enzyme Assays

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The hippocampus was rinsed with cold saline. The tissue was homogenized in prechilled Mito-Cyto solution (Mitochondria Isolation Kit, C0010-50, Applygen Technologies Inc., Shenzhen, China) for mitochondrial extraction. The homogenate was then centrifuged at 800 × g for 10 minutes at 4°C, and the supernatant was collected and re-centrifuged at 12,000 × g for 10 minutes at 4°C. The pellet was then resuspended in Mito-Cyto solution. The procedures for the quantitative detection of SDH (A022-1-1, Nanjing Jiancheng Bioengineering Institute, Succinate Dehydrogenase Assay Kit, Nanjing, China) and Na⁺-K⁺-ATPase activity (Nanjing Jiancheng Bioengineering Institute, Na⁺-K⁺-ATPase assay kit, A070-2-2) were in strict accordance with the manufacturer’s instructions.

Xu X.X., Shi R.X., Fu Y., Wang J.L., Tong X., Zhang S.Q., Wang N., Li M.X., Tong Y., Wang W., He M., Liu B.Y., Chen G.L, & Guo F. (2022). Neuronal nitric oxide synthase/reactive oxygen species pathway is involved in apoptosis and pyroptosis in epilepsy. Neural Regeneration Research, 18(6), 1277-1285.

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Quantifying Na+/K+-ATPase Activity in HepG2 Cells

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Na⁺/K⁺-ATPase activity was identified as the difference between inorganic phosphates (Pi) released and determined using a Na⁺/K⁺-ATPase assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Briefly, following treatment with SWNHs in 37°C for 48 h, HepG2 cells were harvested and physiological saline was added. After three rounds of repeated freezing (−80°C) and thawing, the suspensions of homogenates were centrifuged at 8,000 × g and 4°C for 15 min to obtain the supernatant and then the protein concentration was evaluated using BCA protein assay (Thermo Fisher Scientific, Inc.). Finally, the Na⁺/K⁺-ATPase activity was determined by measuring the amount of inorganic phosphate with malachite green dye, and the results were expressed as micromoles per milligrams of protein.

Li B., Chen X., Yang W., He J., He K., Xia Z., Zhang J, & Xiang G. (2018). Single-walled carbon nanohorn aggregates promotes mitochondrial dysfunction-induced apoptosis in hepatoblastoma cells by targeting SIRT3. International Journal of Oncology, 53(3), 1129-1137.

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Quantifying Na/K-ATPase Activity in HK2 Cells

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The activity of NKA in HK2 cells was detected with the Na/K-ATPase assay kit (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer's specification. The protein concentration was determined with the BCA protein assay kit (Solarbio, China). The activity of NKA was expressed as U per milligram protein.

Li Y., Lu X., Yu Z., Wang H, & Gao B. (2023). Meta-data analysis of kidney stone disease highlights ATP1A1 involvement in renal crystal formation. Redox Biology, 61, 102648.

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Digestive Enzyme Activities in Fish

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After recording of body measurements, 45 fish were collected from each group. The hepatopancreas and gut of fish were removed, frozen in liquid nitrogen and then preserved at −80°C for further analysis. For digestive enzyme analyses, tissue samples were homogenized in ice-cold physiological saline and the supernatant was retained after centrifugation at 3,200 × g for 20 min. The activities of lipase were evaluated using phenolphthalein indicator and the activities of protease were determined by the Folin-phenol reagent method. In addition, the alkaline phosphatase (AKP) assay kit (Nanjing Jiancheng, Nanjing, Jiangsu, China) were used to evaluate the AKP activities, and Na⁺/K⁺-ATPase activities were assessed using a Na⁺/K⁺-ATPase assay kit (Nanjing Jiancheng, Nanjing, China).

He M., Wang K., Liang X., Fang J., Geng Y., Chen Z., Pu H., Hu Y., Li X, & Liu L. (2017). Effects of dietary vitamin E on growth performance as well as intestinal structure and function of channel catfish (Ictalurus punctatus, Rafinesque 1818). Experimental and Therapeutic Medicine, 14(6), 5703-5710.

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