Sc 45044

Manufactured by Santa Cruz Biotechnology

The sc-45044 is a laboratory equipment product offered by Santa Cruz Biotechnology. It serves as a core device for standard laboratory procedures. Detailed specifications and intended use are not provided to maintain an unbiased and factual approach.

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Lab products found in correlation

Anti fibronectin ab2413, by Abcam (1 mentions) Anti hur sc 5261, by Santa Cruz Biotechnology (1 mentions) Anti gapdh g8795, by Merck Group (1 mentions) Np0303box, by Thermo Fisher Scientific (1 mentions) P acc, by Cell Signaling Technology (1 mentions) Sc 45045, by Santa Cruz Biotechnology (1 mentions) Lc2001, by Thermo Fisher Scientific (1 mentions) Hif 1α, by Cell Signaling Technology (1 mentions) P8340, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Image lab software, by Bio-Rad (1 mentions) Chemidoc xr, by Bio-Rad (1 mentions)

2 protocols using sc 45044

Immunoblotting Analysis of Cellular Proteins

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Cells or tissues were collected/homogenized and lysed on ice in an RIPA buffer (25 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol) with protease inhibitors (#88660SPCL, Thermo Fisher Scientific) and a phosphatase inhibitor mix (sc-45044, Santa Cruz). Immunoblotting was performed by probing with the following antibodies: anti-Nox4 (sc-30141), anti-Lamin (sc-376248) and anti-HuR (sc-5261) from Santa Cruz; monoclonal anti-GAPDH (G8795) from Sigma; and anti-fibronectin (ab2413) and anti-SMA (ab5694) from Abcam.

Shi Q., Lee D.Y., Féliers D., Abboud H.E., Bhat M.A, & Gorin Y. (2020). Interplay between RNA-binding protein HuR and Nox4 as a novel therapeutic target in diabetic kidney disease. Molecular Metabolism, 36, 100968.

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Western Blot Analysis of Metabolic Regulators

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Protein lysates were made using MT lysis buffer (Sigma C3228) in the presence of protease (Sigma P8340) and phosphatase inhibitors (Santa Cruz sc-45044 and sc-45045) according to manufacturer’s instructions. Protein concentrations were determined using Bradford assays (BioRad 5000006) and 20–40ug of protein were run on 10% bis-tris gels (ThermoFisher NP0303BOX) and then transferred to nitrocellulose membranes (ThermoFisher LC2001). Blots were probed for antibodies specific for ACC (Cell Signaling 3676S), p-ACC (Cell Signaling 11818S), AMPK (Cell Signaling 2532S), p-AMPK (Cell Signaling 2535S), AR (Sigma 06–680), HIF-1α (Cell Signaling 3716) and β-Actin (Sigma A2228). Secondary incubations were performed with HRP-antimouse (Santa Cruz SC-516102) and HRP-anti rabbit (Enzo ADI-SAB-300-J). Primary antibody incubations were done overnight at 4°C and secondary antibody incubations were done for 1h at room temperature. HRP was developed (BioRad 170–5061) and recorded using a Bio-Rad imager (BioRad ChemiDoc XRS+) and quantified using ImageLab software (Bio-Rad, Hercules, California).

Consiglio C.R., Udartseva O., Ramsey K.D., Bush C, & Gollnick S.O. (2020). Enzalutamide, an androgen receptor antagonist, enhances myeloid cell-mediated immune suppression and tumor progression. Cancer immunology research, 8(9), 1215-1227.

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