Tissuefaxs plus s

A portion of prefixed liver tissue in 4% formalin was removed, embedded in paraffin, and sectioned at a thickness of 5 μm. These sections were then stained with hematoxylin and eosin (H&E). Another portion was embedded in Tissue-Tek O.C.T. Compound (4583, SAKURA, Seattle, WA, USA), cryosectioned, and stained with Oil Red O (O1391, Sigma-Aldrich, USA). Examination, observation, and imaging were performed using the panoramic tissue cell quantification system (TissueFAXS Plus S, TissueGnostics, Vienna, Austria).

After reperfusion with normal saline, the isolated mouse heart was weighed, embedded in the optimal cutting temperature compound medium (Sakura Finetek, Japan), then frozen in liquid nitrogen for a few seconds and stored in -80° refrigerator. The heart was sectioned into 6-μm thick slices with a freezing microtome (CM1950, Leica, China). The heart slices were fixed in 4% paraformaldehyde at room temperature for 10 min, washed with running water for 2 min, and stained with hematoxylin-eosin (HE) staining kit (Solarbio, Beijing, China) following the manufacturer’s instructions. The section images were taken with TissueFAXS Plus S (Tissue Gnostics, Austria).

Safranin O is a cationic dye that binds polyanions and binds to GAGs in chondrocytes to give them a red color, with the intensity of the red color being directly proportional to the GAG content. It is used to assess the content of GAGs in chondrocytes and can reflect the ability of chondrocytes to perform anabolic and catabolic activities. We washed the treated chondrocytes with PBS and fixed them with 4% paraformaldehyde for 20 min, then washed them with PBS and incubated them with safranin O staining solution (Solarbio, Beijing, China) for 30 min at room temperature. The cells were then washed with PBS and observed by microscopy (Tissue FAXS Plus S; Tissue Gnostics), and photographs were taken.