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2 protocols using hyperoside

1

Hyperoside Modulation of Inflammatory Pathways

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Hyperoside (purity ≥ 98%, Lot. No. JOT-10,193) was ordered from Chengdu Pufei De Biotech Co., Ltd (China). 2-Aminoethyl diphenylborinate (2-APB) and lipopolysaccharides (LPS) were obtained from Sigma-Aldrich (USA). Calf thymus DNA (ctDNA), Lipofectamine 2000, Dulbecco’s Modified Eagle’s Medium (DMEM), and penicillin/streptomycin were products from Thermo Fisher Scientific (USA). Fetal bovine serum (FBS) was ordered from Nobimpex (Germany). 2′,3′-Cyclic guanosine monophosphate–adenosine monophosphate (cGAMP) sodium and 5,6-dimethyl-9-oxo-9 H-xanthene-4-acetic acid (DMXAA) were products from MedChemExpress (USA). Evans blue dye was purchased from Solarbio (China).
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2

HPLC Analysis of Bioactive Compounds

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Six commercial standards of HPLC grade, including catechins, chlorogenic acid, hyperoside, quercetin, kaempferol, and isorhamnetin, were purchased from Chengdu Pufei De Biotech Co., Ltd. (China). Other HPLC grade reagents used were acetonitrile and methanol from Thermo Fisher Scientific Co., Ltd. (USA). The Watsons distilled water was applied to prepare for samples and the mobile phase. All other reagents were just met for an analytical grade.
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